Resources

The Proteomics Facility is housed in dedicated instrumentation laboratories located in MBB 1.420 and PHR 5.220.

The following instruments are included in the facility with installation date in parantheses.


LC-ESI-MS/MS

MBB 1.420

Two Thermo Orbitrap Fusions, one with ETD fragmentation coupled to  Dionex Ultimate 3000 RSLCnano Nanoflow UPLCs.

Thermo Scientific

MS and CID or HCD MSn analysis with electrospray ionization. Protein identification from gel bands, gel sections, and complex mixtures. Proteome Discoverer, X!Tandem, and MASCOT database search engines, Scaffold data visualization, validation, quantitation with spectral counting or stable isotope labeling. Post-translational modification site determination.

 


Protein Digest

MBB 1.420

Intavis DigestPro MSi (2016)

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The Intavis DigestPro MSi is an automated robotic platform for performing enzymatic digests of protein samples submitted to the lab. Along with fully automated in-gel and in-solution digest capabilities, the DigestPro can also perform automated ziptipping procedures, which has higher reproducibility in comparison to manual ziptip procedures.


MALDI-MS

AB Voyager-DE PRO MALDI-TOF (2001)

Maldi Workstation

MALDI-MS  for biomolecules.
Self-service training available for Voyager.

More information at: MALDI-MS


LC Systems

MBB 1.420

Dionex Ultimate 3000 UPLC (2015)

Ultimate 3000
Biorad BioLogic DuoFlow FPLC (2 systems-room temp. and 4 C) (2007)
Dionex LC Packings nanoLC (2004)
Self-service FPLC for high resolution purification of proteins and peptides when recovery of activity is important.
Flow rates ul/min-ml/min.

More information at: LC Systems


 

Luminometer

MBB 1.420

Berthold Technologies Mithras LB 940 Luminometer (2005)

img_4716

Measures chemiluminescence in solution for alkaline phosphatase or luciferase assays.

More information at: Luminometer MITHRAS


Protein/Peptide Concentration Measurement

EMD Millipore Direct Detect IR Spectrometer (2012)

Uses FTIR to quantitate proteins or peptides in solution without interference from reagents (most detergents etc)


 

Gel Electrophoresis

MBB 1.420

Gel Electrophoresis

SDS-PAGE

Training and space provided for self-service gel electrophoresis


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