TTP has expertise in assisting investigators in design and optimization of target-based biochemical and cell-based assays applicable for robust compound screening. TTP executes small molecule screens to identify chemical probes for potential lead optimization, analyzes data and report of analyzed data to investigators. Advice and/or services regarding the delineation of the modality of inhibition by hit compounds are also offered.
TTP offers a wide variety of detection modalities for assay measurements including:
- Absorbance, Fluorescence, TRF, FRET, FP, Luminescence, Alphascreen, Scintillation count, Circular Dichroism.
- Spectra and well area scan/End-point or kinetic measurement.
- Cellular imaging (DAPI, GFP, RFP, CFP, YFP; 4x, 10x, 20x, 40x)
TTP is supporting traditional biochemical-based HTS campaigns (homogeneous, i.e., “mix and read”) as well as cell-based and other types of heterogeneous assays (ELISA or bead-based assays) with well-equipped fully automated workstations compatible with 96-, 384- and 1,536-well plate formats. These systems have a combined operating capacity of approximately 50,000 wells per day (384-well format). TTDDDP supports diverse assay strategies such as, but not limited to, binding, reporter assays, enzymatic, cell counting, viability, proliferation or cytotoxicity.