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Lambowitz Lab
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  • Research Summary
    • (A) Mobile Group II Intron, Evolutionary Ancestors of Retroviruses, Retrotransposons, and Spliceosomal Introns in Eukaryotes.
    • (B) Group II Intron Reverse Transcriptases
    • (C) Reverse Transcriptases Associated with CRISPR-Cas Systems and Other Bacterial and Organellar Reverse Transcriptases
    • (D) Thermostable Group II Intron Reverse Transcriptases (TGIRTs) and Their Applications in High Throughput RNA Sequencing (RNA-Seq).
    • (E) Analysis of Extracellular RNAs, RNA Diagnostics, and Liquid Biopsy and tRNA fragments
  • People
    • Principal Investigator
    • Assistant to Dr. Lambowitz
    • Postdoctoral Associates
    • Research Engineering/Scientist Associates
    • Research Associates
    • Graduate Students
    • Laboratory Assistants
  • Publications
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  • Targetron Design
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Lambowitz Lab

Department of Molecular Biosciences

100 East 24th St., Stop A5000
Austin, TX 78712 (email)

2005

December 13, 2024, Filed Under: Publications

Domain structure and three-dimensional model of a group II intron-encoded reverse transcriptase.

Citation:

Blocker FJH, Mohr G, Conlan LH, Qi L, Belfort M, Lambowitz AM. Domain structure and three-dimensional model of a group II intron-encoded reverse transcriptase. RNA. 11 (1) :14-28.

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December 13, 2024, Filed Under: Publications

Number, position, and significance of the pseudouridines in the large subunit ribosomal RNA of Haloarcula marismortui and Deinococcus radiodurans.

Citation:

Del Campo M, Recinos C, Yanez G, Pomerantz SC, Guymon R, Crain PF, McCloskey JA, Ofengand J. Number, position, and significance of the pseudouridines in the large subunit ribosomal RNA of Haloarcula marismortui and Deinococcus radiodurans. RNA. 11 (2) :210-9.

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December 13, 2024, Filed Under: Publications

The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function.

Citation:

Huang H-R, Rowe CE, Mohr S, Jiang Y, Lambowitz AM, Perlman PS. The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function. Proc Natl Acad Sci U S A. 102 (1) :163-8.

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December 13, 2024, Filed Under: Publications

A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface.

Citation:

Paukstelis PJ, Coon R, Madabusi L, Nowakowski J, Monzingo A, Robertus J, Lambowitz AM. A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface. Mol Cell. 17 (3) :417-28.

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December 13, 2024, Filed Under: Publications

A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles.

Citation:

Zhao J, Lambowitz AM. A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proc Natl Acad Sci U S A. 102 (45) :16133-40.

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December 13, 2024, Filed Under: Publications

Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library.

Citation:

Yao J, Zhong J, Lambowitz AM. Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library. Nucleic Acids Res. 33 (10) :3351-62.

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December 13, 2024, Filed Under: Publications

Recruitment of host functions suggests a repair pathway for late steps in group II intron retrohoming.

Citation:

Smith D, Zhong J, Matsuura M, Lambowitz AM, Belfort M. Recruitment of host functions suggests a repair pathway for late steps in group II intron retrohoming. Genes Dev. 19 (20) :2477-87.

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December 13, 2024, Filed Under: Publications

Retargeting mobile group II introns to repair mutant genes.

Citation:

Jones JP, Kierlin MN, Coon RG, Perutka J, Lambowitz AM, Sullenger BA. Retargeting mobile group II introns to repair mutant genes. Mol Ther. 11 (5) :687-94.

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December 13, 2024, Filed Under: Publications

Retrotransposition strategies of the Lactococcus lactis Ll.LtrB group II intron are dictated by host identity and cellular environment.

Citation:

Coros CJ, Landthaler M, Piazza CL, Beauregard A, Esposito D, Perutka J, Lambowitz AM, Belfort M. Retrotransposition strategies of the Lactococcus lactis Ll.LtrB group II intron are dictated by host identity and cellular environment. Mol Microbiol. 56 (2) :509-24.

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Recent Publications

Structural basis for the evolution of a domesticated group II intron–like reverse transcriptase to function in host cell DNA repair

Tagged With: 2025

Citation: Park SK, Guo M, Stamos JL, Wantae K, Lee S, Zhang YJ, Lambowitz AM. Structural basis of the evolution of a domesticated group II intron-like reverse transcriptase to function in host cell DNA repair. Biochemistry. 2025 July 29; 12231):e2504208122. Download Citation

Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers

Tagged With: 2024

Citation: Yao J, Xu H, Ferrick-Kiddie EA, Nottingham RM, Wu DC, Ares M, Lambowitz AM. Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers. PloS Genet. 2024 Sep 26; 20(9):e1011416 185 (20) :3671-3688. Download Citation

Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2

Tagged With: 2023

Citation: Cui H, Diedrich JK, Wu DC, Lim JJ, Nottingham RM, Moresco JJ, Yates JR, Blencowe BJ, Lambowitz AM, Schimmel P. Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2. Nature Cell Biology [Internet]. 25 :592–603. Download Citation cui_2023.pdf 10.53 MB Full Text News & views highlight:… read more 

Group II-like Reverse Transcriptases Function in Double Strand Break Repair

Tagged With: 2022

Citation: Park SK, Mohr G, Yao J, Russell R, Lambowitz AM. Group II-like Reverse Transcriptases Function in Double Strand Break Repair. Cell [Internet]. 185 (20) :3671-3688. Download Citation park_cell_2022.pdf 12.54 MB Full Text Cell Leading Edge Highlight: Moran, J.V. and Wilson, T.E. Reverse transcriptase meets DNA, again: Possible roles for transposable… read more 

Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling

Tagged With: 2020

Citation: Yao J, Wu DC, Nottingham RM, Lambowitz AM. Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling. eLife [Internet]. Download Citation Last updated on 09/15/2020

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