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Lambowitz Lab
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  • Research Summary
    • (A) Mobile Group II Intron, Evolutionary Ancestors of Retroviruses, Retrotransposons, and Spliceosomal Introns in Eukaryotes.
    • (B) Group II Intron Reverse Transcriptases
    • (C) Reverse Transcriptases Associated with CRISPR-Cas Systems and Other Bacterial and Organellar Reverse Transcriptases
    • (D) Thermostable Group II Intron Reverse Transcriptases (TGIRTs) and Their Applications in High Throughput RNA Sequencing (RNA-Seq).
    • (E) Analysis of Extracellular RNAs, RNA Diagnostics, and Liquid Biopsy and tRNA fragments
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    • Principal Investigator
    • Assistant to Dr. Lambowitz
    • Postdoctoral Associates
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Lambowitz Lab

Department of Molecular Biosciences

100 East 24th St., Stop A5000
Austin, TX 78712 (email)

2008

December 13, 2024, Filed Under: Publications

Function of the C-terminal domain of the DEAD-box protein Mss116p analyzed in vivo and in vitro.

Citation:

Mohr G, Del Campo M, Mohr S, Yang Q, Jia H, Jankowsky E, Lambowitz AM. Function of the C-terminal domain of the DEAD-box protein Mss116p analyzed in vivo and in vitro. J Mol Biol. 375 (5) :1344-64.

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December 13, 2024, Filed Under: Publications

Group II intron protein localization and insertion sites are affected by polyphosphate.

Citation:

Zhao J, Niu W, Yao J, Mohr S, Marcotte EM, Lambowitz AM. Group II intron protein localization and insertion sites are affected by polyphosphate. PLoS Biol. 6 (6) :e150.

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December 13, 2024, Filed Under: Publications

Group II intron-based gene targeting reactions in eukaryotes.

Citation:

Mastroianni M, Watanabe K, White TB, Zhuang F, Vernon J, Matsuura M, Wallingford J, Lambowitz AM. Group II intron-based gene targeting reactions in eukaryotes. PLoS One. 3 (9) :e3121.

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December 13, 2024, Filed Under: Publications

Identification and evolution of fungal mitochondrial tyrosyl-tRNA synthetases with group I intron splicing activity.

Citation:

Paukstelis PJ, Lambowitz AM. Identification and evolution of fungal mitochondrial tyrosyl-tRNA synthetases with group I intron splicing activity. Proc Natl Acad Sci U S A. 105 (16) :6010-5.

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December 13, 2024, Filed Under: Publications

Structure of a tyrosyl-tRNA synthetase splicing factor bound to a group I intron RNA.

Citation:

Paukstelis PJ, Chen J-H, Chase E, Lambowitz AM, Golden BL. Structure of a tyrosyl-tRNA synthetase splicing factor bound to a group I intron RNA. Nature. 451 (7174) :94-7.

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December 13, 2024, Filed Under: Publications

A three-dimensional model of a group II intron RNA and its interaction with the intron-encoded reverse transcriptase.

Citation:

Dai L, Chai D, Gu S-Q, Gabel J, Noskov SY, Blocker FJH, Lambowitz AM, Zimmerly S. A three-dimensional model of a group II intron RNA and its interaction with the intron-encoded reverse transcriptase. Mol Cell. 30 (4) :472-85.

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December 13, 2024, Filed Under: Publications

DEAD-box proteins can completely separate an RNA duplex using a single ATP.

Citation:

Chen Y, Potratz JP, Tijerina P, Del Campo M, Lambowitz AM, Russell R. DEAD-box proteins can completely separate an RNA duplex using a single ATP. Proc Natl Acad Sci U S A. 105 (51) :20203-8.

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December 13, 2024, Filed Under: Publications

Toward predicting self-splicing and protein-facilitated splicing of group I introns.

Citation:

Vicens Q, Paukstelis PJ, Westhof E, Lambowitz AM, Cech TR. Toward predicting self-splicing and protein-facilitated splicing of group I introns. RNA. 14 (10) :2013-29.

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Recent Publications

Structural basis for the evolution of a domesticated group II intron–like reverse transcriptase to function in host cell DNA repair

Tagged With: 2025

Citation: Park SK, Guo M, Stamos JL, Wantae K, Lee S, Zhang YJ, Lambowitz AM. Structural basis of the evolution of a domesticated group II intron-like reverse transcriptase to function in host cell DNA repair. Biochemistry. 2025 July 29; 12231):e2504208122. Download Citation

Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers

Tagged With: 2024

Citation: Yao J, Xu H, Ferrick-Kiddie EA, Nottingham RM, Wu DC, Ares M, Lambowitz AM. Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers. PloS Genet. 2024 Sep 26; 20(9):e1011416 185 (20) :3671-3688. Download Citation

Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2

Tagged With: 2023

Citation: Cui H, Diedrich JK, Wu DC, Lim JJ, Nottingham RM, Moresco JJ, Yates JR, Blencowe BJ, Lambowitz AM, Schimmel P. Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2. Nature Cell Biology [Internet]. 25 :592–603. Download Citation cui_2023.pdf 10.53 MB Full Text News & views highlight:… read more 

Group II-like Reverse Transcriptases Function in Double Strand Break Repair

Tagged With: 2022

Citation: Park SK, Mohr G, Yao J, Russell R, Lambowitz AM. Group II-like Reverse Transcriptases Function in Double Strand Break Repair. Cell [Internet]. 185 (20) :3671-3688. Download Citation park_cell_2022.pdf 12.54 MB Full Text Cell Leading Edge Highlight: Moran, J.V. and Wilson, T.E. Reverse transcriptase meets DNA, again: Possible roles for transposable… read more 

Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling

Tagged With: 2020

Citation: Yao J, Wu DC, Nottingham RM, Lambowitz AM. Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling. eLife [Internet]. Download Citation Last updated on 09/15/2020

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