Mark Ruszczycky

Mark RuszczyckyDegree: B.S., University of California, Riverside
M.S., University of California, Riverside
M.D./Ph.D. Case Westrern Reserve

Entered Group: Summer, 2008

Research Projects: Mechanistic Studies of Novel Enzyme Catalysis


Group Publications:

Szu, P.; Ruszczucky, M.; Choi, S. H.; Yan, F.; Liu, H.-w. “Characterization and Mechanistic Studies of DesII: A Radical S-Adenosylmethionine Enzyme Involved in the Biosynthesis of TDP-D-Desosamine.” J. Am. Chem. Soc. 2009, 131, 14030-14042.

Ruszczycky, M. W.; Choi, S. H.; Liu, H.-w. “Stoichiometry of the Redox Neutral Deamination and Oxidative Dehydrogenation Reactions Catalyzed by the Radical SAM Enzyme DesII.” J. Am. Chem. Soc.2010, 132, 2359-2369.

Kim, H. J.; Ruszczycky, M. W.; Choi, S. H.; Liu, Y.-n.; Liu, H.-w. “An Enzyme-Catalyzed [4+2] Cycloaddition is a Key Step in the Biosynthesis of Spinosyn A.” Nature 2011, 473, 109-112.

Ruszcycky, M. W.; Choi, S.-h.; Mansoorabadi, S. O.; Liu, H.-w. “Mechanistic Studies of the Radical SAM Enzyme DesII: EPR Characterization of a Radical Intermediate Generated During Its Catalyzed Dehydrogenation of TDP-D-Quinovose.” J. Am. Chem. Soc. 2011, 133, 7292-7295.

Choi, S.-h.; Ruszcycky, M. W.; Zhang, H.; Liu, H.-w. “A Fluoro Analogue of UDP-α-D-Glucuronic Acid is an Inhibitor of UDP-α-D-Apiose/UDP-α-D-Xylose Synthase.” Chem. Comm. 2011, 10130-10132.

Sun, H. G.; Ruszczycky, M. W.; Chang, W.-c.; Thibodeaux, C. J.; Liu, H.-w. “Nucleophilic Participation of the Reduced Flavin Cofactor in the Mechanism of UDP-Galactopyranose Mutase.” J. Biol. Chem. 2012,287, 4602-4608.

Ruszczycky, M. W.; Ogasawara, Y.; Liu, H.-w. “Radical SAM Enzymes in the Biosynthesis of Sugar-Containing Natural Products.” Biochem. Biophys. Acta Proteins & Proteomics, 2012, 1824, 1231-1244.

Kim, H. J.; Ruszczycky, M. W.; Liu, H.-w. “Current Development and Challenges in the Search of a Naturally Selected Diels-Alderase.” Cur. Opin. Chem. Biol. 2012, 16, 124-131.

Ruszczycky, M. W.; Choi, S. H.; Liu, H.-w. “A Combined EPR-Kinetic Isotope Effect Study of Radical-Mediated Dehydrogenation of an Alcohol by the Radical SAM Enzyme DesII: Evidence for General Base Catalysis. ” Proc. Natl. Acad. Sci. USA 2013, 110, 2088-2093

Ko, Y.; Ruszczycky, M. W.; Liu, H.-w. “Mechanistic Studies of the Radical SAM Enzyme DesII with TDP-D-fucose.” Angew. Chem. Int. Ed. 2015, 54, 860-863.

Ruszczycky, M. W.; Liu, H.-w. “Mechanistic Enzymology of the Radical SAM Enzyme DesII.” Israel. J. Chem. 2015, 55, 315-324.

Lin, G.-m.; Choi, S.-h.; Ruszczycky, M. W.; Liu, H.-w. “Mechanistic Investigation of the Radical S-Adenosyl-methionine Enzyme DesII Using Fluorinated Analogs.” J. Am. Chem. Soc. 2015, 137, 4964-4967.

Liu, C.-H.; Wang, S.-A.; Ruszczycky, M. W.; Chen, H.; Li, K.; Murakami, K.; Liu, H.-w. “Studies of 1-Amino-2,2-difluorocyclopropane-1-carboxylate: Mechanism of Decomposition and Inhibition of ACC Deaminase.” Org. Lett. 2015, 17, 3342-3345.

Jeon, B.-s.; Wang, S.-A.; Ruszczycky, M. W.; Liu, H.-w. “Natural [4+2]-Cyclases.” Chem. Review. 2016, submitted.