Degree: B.S., National Taiwan University, Taiwan
Entered Group: Fall, 2016 (Chemistry program)
Research Projects: Mechanistic studies of the Biosynthesis of Secondary Metabolites
Group Publications:
Romo, A.; Ikeuchi, H.; Shiraishi, T.; Lin, G.-M.; Geng, Y.; Lee, Y.-H.; Liem, P. H.; Ma, T.; Ogasawara, Y.; Shin-Ya, K.; Nishiyama, M.; Kuzuyama, T.; Liu, H.-w. “Miharamycins and Amipurimycin Biosynthetic Gene Clusters: Unravelling the Origins of 2-Aminopurinyl Peptidyl Nucleoside Antibiotics.” J. Am. Chem. Soc. 2019, 141, 14152-14159. [PMC6774755].
Zhong, A.; Lee, Y.-H.; Liu, Y.-n.; Liu, H.-w. “Biosynthesis of Oxetanocin A Includes a B12-dependent Radical SAM Enzyme Able to Catalyze both Oxidative Ring Contraction and the Demethylation of SAM.” Biochemistry. 2021, 60, 537-546. [PMC7904626]
Lee, Y.-H.; Liu, H.-w. “Studies of GenK and OxsB, Two B12-Dependent Radical SAM Enzymes Involved in Natural Product Biosynthesis.” Meth. Enzymol. 2022, 667, 71-90. [NIHMSID1788471]
Lee, Y.-H.; Hou, X.; Chen, R.; Feng, J.; Liu, X.; Ruszczycky, M. W.; Guo, J.-M.; Wang, B.; Zhou, J.; Liu, H.-w. “Radical S-Adenosyl Methionine Enzyme BlsE Catalyzes a Radical Mediated 1,2-Diol Dehydration during the Biosynthesis of Blasticidin S.” J. Am. Chem. Soc. 2022, 144, 4478-4486.[PMC8934444]
Ren, D.; Lee, Y.-H.; Wang, S.-A.; Liu, H.-w. “Characterization of the Oxazinomycin Biosynthetic Pathway Revealing the Key Role of a Non-heme Iron-dependent Monooxygenase.” J. Am. Chem. Soc. 2022, 144, 10968-10977. [PMC9233086]
Fan, P.-H.; Geng, Y.; Romo, A. J.; Zhong, A.; Zhang, J.; Yeh, Y.-C.; Lee, Y.-H.; Liu, H.-w. “Two Radical SAM Enzymes are Necessary and Sufficient for the In Vitro Production of the Oxetane Nucleoside Antiviral Agent Albucidin.” Angew. Chem. Int. Ed. 2022, 61, e202210362. [PMC9561071]
Lee, Y-H.; Ren, D.; Jeon, B-s.; Liu, H.-w. “S-Adenosylmethionine: More than Just a Methyl Donor.” Nat. Prod. Rep. 2023, 40, 1521-1549. [PMC10491745]
Lee, Y.-H.; Yeh, Y.-C.; Fan, P.-H.; Zhong, A.; Ruszczycky, M. W.; Liu, H.-w. “Changing Fates of the Substrate Radicals Generated in the Active Sites of the B12-dependent Radical SAM Enzymes OxsB and AlsB.” J. Am. Chem. Soc. 2023, 145, 3656-3664. [PMC9940012]
Zheng, Z.; Lee, Y.-H.; Ruszczycky, M. W.; Ushimaru, R.; Liu, H.-w. “Twitch Radical S-Adenosyl L-Methionine Alcohol Dehydrogenases.” Encyclopedia Inorg. Bioinorg. Chem. 2024, eibc2869. [NIHMSID2015061]
Zheng, Z.; Xiong, J.; Bu, J.; Ren, D.; Lee, Y.-H.; Yeh, Y.-C.; Lin, C.-I.; Parry, R.; Guo, Y.; Liu, H.-w. “Reconstitution of the Final Steps in the Biosynthesis of Valanimycin Reveals the Origin of its Characteristic Azoxy Moiety.” Angew. Chem. Int. Ed. 2024, 63, e202315844. [PMC10843709]
Ren, D.; Lee, Y.-H.; Liu, H.-w. “Expression, Purification and Characterization of Non-heme Iron-dependent Monooxygenase OzmD in Oxazinomycin Biosynthesis.” Meth. Enzymol. 2024, 704, 113-142.
Lee, Y-H.; Ren, D.; Chen. R.; Liu, X.; Engstrom, A.; Liu, H.-w. “Blasticidin S Biosynthesis Involves a Unique Interplay between a Radical SAM Dehydratase, a Transaminase and an ATP-Grasp Ligase in Order to Avoid a Futile Cycle.” ACS Catalysis 2025, 15, 2742-2749. [NIHMSID2055467]
Ma, B.; Lee, Y.-H.; Ruszczycky, M. W.; Ren, D.; Engstrom, A.; Liu, H.-w.; Tao, L. “EPR Characterization of the BlsE Substrate Radical Offers Insight into the Determinants of Reaction Outcome that Distinguish Radical SAM Dioldehydratases from Dehydrogenases.” J. Am. Chem. Soc. 2025, 147, 4111-4119. [PMC11826333]