| Pilot Set I | Pilot Set II | Pilot Set III |
| No. | Gene ID | Gene Name | Source * | EST ID | Stock ID | Sequence Verification ** |
| 1 | At1g56010 | NAC1 | cDNA (TAMU) | + | ||
| 2 | At3g49530 | NAC2 | cDNA (TAMU) | + | ||
| 3 | At1g53230 | TCP3 | cDNA (TAMU) | + | ||
| 4 | At1g53260 | DAG | cDNA (TAMU) | + | ||
| 5 | At1g53250 | Hypothetical protein3 | cDNA (TAMU) | + | ||
| 6 | At1g53240 | MNDMD | cDNA (TAMU) | + | ||
| 7 | At1g53210 | Hypothetical protein2 | cDNA (TAMU) | + | ||
| 8 | At1g53200 | Hypothetical protein | cDNA (TAMU) | + | ||
| 9 | At1g53190 | Ring finger protein | cDNA (TAMU) | + | ||
| 10 | At1g56360 | Unknown gene-814 | cDNA (TAMU) | + | ||
| 11 | Non coding RNA | cDNA (TAMU) | + | |||
| 12 | At1g79740 | Transposase | cDNA (TAMU) | + | ||
| 13 | At5g58140 | Kinase | cDNA (TAMU) | + | ||
| 14 | At4g36650 | Transcription initiation factor | cDNA (TAMU) | + | ||
| 15 | At3g50670 | U1 snRNP 70K protein | ABRC | AA394496 | 130B1XP | + |
| 16 | At3g53500 | Splicing factor Arg/Ser rich 7 | ABRC | T22516 | 105L5T7 | + |
| 17 | At3g55200 | Splicesomal protein SAP 130 | ABRC | T42903 | 114O17T7 | + |
| 18 | At3g60610 | Splicing factor | ABRC | Z35043 | ATTS3682 | + |
| 19 | At3g61240 | DEAD box RNA helicase RH12 | ABRC | Z30728 | ATTS2474 | + |
| 20 | At3g61860 | Arg/Ser-rich splicing factor RSP31 | ABRC | T14106 | 47B7T7 | + |
| 21 | At4g02430 | Alternative splicing factor ASF | ABRC | Z25964 | ATTS1241 | + |
| 22 | At4g02840 | Small nuclear riboprotein Sm-D1 protein | ABRC | T46269 | 139I4T7 | + |
| 23 | At4g03430 | Splicing factor | ABRC | AA394697 | 157A2XP | – |
| 24 | At4g16630 | ATP dependent RNA helicase DRS | gDNA (hlcrDRS) | Z26774 | ||
| 25 | At4g18460 | RNA helicase Cdc28p | ABRC | H76018 | 193J17T7 | + |
| 26 | At4g21660 | Splicesome associated protein | ABRC | AA651260 | 178J20XP | + |
| 27 | At4g25500 | Splicing factor At-SRp40 | ABRC | H37668 | 185A17T7 | + |
| 28 | At4g25630 | Fibrillarin protein Sb21 | gDNA (Fbllrn) | Z17495 | ||
| 29 | At4g30220 | snRNP Sm-F protein | ABRC | AA712720 | 193I11T7 | + |
| 30 | At4g31580 | RSZp22 splicing factor | ABRC | N65843 | 240D15T7 | + |
| 31 | At4g36690 | Splicing factor U2AF large chain | ABRC | N96704 | G3A11T7 | + |
| 32 | At4g34910 | RNA helicase RH16 | ABRC | T46809 | 143L17T7 | + |
| 33 | At4g37120 | Step II splicing factor | ABRC | H36844 | 179N2T7 | + |
| 34 | At4g38780 | Splicing factor Prp8 | ABRC | T14115 | 47D7T7 | + |
| 35 | At5g13010 | Splicng factor ATP-dependent RNA helicase | ABRC | AA651273 | 179C6XP | + |
| 36 | At5g09690 | MRS2 protein | ABRC | T22663 | 105N2T7 | + |
| 37 | At4g38780 | Splicing factor Prp8-exon1 | gDNA (Prp8-ex1) | |||
| 38 | At4g38780 | Splicing factor Prp8-exon2 | gDNA (Prp8-ex2) | |||
| 39 | At4g38780 | Splicing factor Prp8-exon3 | gDNA (Prp8-ex3) | |||
| 40 | F17A9.17 | Brassica Control 1 – UDP-glucose:sterol glucosyltransferase | WISC (Brassica) | T04809 | WG1A4 | |
| 41 | At2g1911 | Brassica Control 2 – putative heavy metal P-type ATPase | WISC (Brassica) | AV530075 | WG1G10 | |
| 42 | F1N18.5 | Brassica Control 3 – T31E10.2 photosystem II type I chlorophyl a/b binding protein | WISC (Brassica) | AV520935 | WG1H5 | |
| 43 | F1N18.6 | Brassica Control 4 – putative photosystem I type I chlorophyl a/b binding protein | WISC (Brassica) | AV521122 | WG2C4 | |
| 44 | F3L17.13 | Brassica Control 5 – putative protein | WISC (Brassica) | T75784 | WG2D6 | |
| 45 | T5E21.11 | Brassica Control 6 – valyl tRNA synthetase (valRS) | WISC (Brassica) | AV526903 | WG2G11 | |
| 46 | At2g2082 | Brassica Control 7 – unknown protein | WISC (Brassica) | AV554725 | WG3G9 | |
| 47 | MBD2.17 | Brassica Control 8 – COP8 homolog – note position verus 139! | WISC (Brassica) | AA394494 | WG3G11 | |
| 48 | At2g3343 | Brassica Control 9 – plastid protein | WISC (Brassica) | AV562631 | WG4D7 | |
| 49 | At2g4607 | Brassica Control 15 – putative mitogen – activated protein kinase | WISC (Brassica) | AV530854 | WG8A9 | |
| 50 | At2g3683 | Brassica Control 16 – putative aquaporin (tonoplast intrinsic protein gamma) | WISC (Brassica) | AV550938 | TG2D6 | |
| 51 | F23N20.1 | Brassica Control 17 – unknown protein | WISC (Brassica) | AV520594 | TG5D9 | |
| 52 | Brassica Control 18 – EC2B3 | WISC (Brassica) | ||||
| 53 | MBD2.18 | Brassica Control 19 – thioredoxin (clone GIF1) | WISC (Brassica) | AV554324 | EC2C7 | |
| 54 | F17I14_1 | Brassica Control 20 – microbody NAD-dependent malate dehydrogenase | WISC (Brassica) | AV525389 | EC2D1 | |
| 55 | NWL2.17 | Brassica Control 21 – predicted pseudogene | WISC (Brassica) | AV562296 | EC2D8 | |
| 56 | At4g01150 | Brassica Control 22 – predicted protein | WISC (Brassica) | AV441139 | EC2D9 | |
| 57 | Brassica Control 23 – EC2D11 | WISC (Brassica) | ||||
| 58 | F4M19_50 | Brassica Control 24 – putative protein | WISC (Brassica) | AV523700 | EC2E12 | |
| 59 | F3C22_150 | Brassica Control 25 – plastid division protein FtsZ mRNA | WISC (Brassica) | AV559837 | EC2F1 | |
| 60 | MLD14.15 | Brassica Control 26 – tyrosine phosphatase – like protein | WISC (Brassica) | AI994436 | EC2H7 | |
| 61 | Brassica Control 27 – EC3B2 | WISC (Brassica) | ||||
| 62 | Brassica Control 28 – EC3B4 | WISC (Brassica) | ||||
| 63 | At4g05320 | Brassica Control 29 – polyubiquitin (ubq10) | WISC (Brassica) | AV544113 | EC3C8 | |
| 64 | At2g40000 | Brassica Control 30 – putative nematode – resistance protein | WISC (Brassica) | AV555067 | EC3D2 | |
| 65 | Brassica Control 31 – hit on BAC T8D3 end – not annotated | WISC (Brassica) | AV547452 | EC3E10 | ||
| 66 | F7G19.24 | Brassica Control 32 – similarity to Picea histone H2A | WISC (Brassica) | T14194 | EC3E12 | |
| 67 | F23M19.3 | Brassica Control 33 – unknown | WISC (Brassica) | BE524433 | EC3F1 | |
| 68 | Brassica Control 34 – EC3F3 | WISC (Brassica) | ||||
| 69 | F14D7.2 | Brassica Control 35 – annexin (AnnAt1) | WISC (Brassica) | AV559022 | EC3F4 | |
| 70 | At2g30410 | Brassica Control 36 – putative TCP1 – chaperonin cofactor A | WISC (Brassica) | T14083 | EC3F12 | |
| 71 | F2K13_150 | Brassica Control 37 – quinone oxidoreductase – like protein | WISC (Brassica) | T21718 | EC3G12 | |
| 72 | MUD21.18 | Brassica Control 38 – pectinesterase like protein | WISC (Brassica) | AV537452 | EC3H4 | |
| 73 | At2g32700 | Brassica Control 39 – unknown protein | WISC (Brassica) | BE526178 | EC4C5 | |
| 74 | Brassica Control 40 – EC4D9 | WISC (Brassica) | ||||
| 75 | At4g12600 | Brassica Conrol 41 – Ribosomal protein L7Ae-like | WISC (Brassica) | T14197 | EC4E7 | |
| 76 | F22O6_220 | Brassica Control 42 – no predicted protein in genome sequence | WISC (Brassica) | AV565053 | EC4E8 | |
| 77 | C7A10.500 | Brassica Control 43 – putative protein | WISC (Brassica) | H76120 | EC4F1 | |
| 78 | F9E10.20 | Brassica Control 44 – unknown protein (T29M8.5) mRNA, complete cds | WISC (Brassica) | AV530446 | EC4F10 | |
| 79 | Brassica Control 45 – no genome region and also weak EST hit | WISC (Brassica) | AV548183 | EC4F11 | ||
| 80 | MDA7.5 | Brassica Control 46 – heat shock protein 90 | WISC (Brassica) | AV439634 | EC4G4 | |
| 81 | MLN21.1 | Brassica Control 47 – transcription factor EREBP – like protein | WISC (Brassica) | AV553751 | EC4G7 | |
| 82 | F28M20.10 | Brassica Control 48 – putative protein similarity to DNA – binding protein ABF2, Avena | WISC (Brassica) | AV536499 | EC4H3 | |
| 83 | F12A12.10 | Brassica Control 49 – putative protein similarity to several oxidases, mainly gibberellin 20-oxidases | WISC (Brassica) | AV561112 | EC4H7 | |
| 84 | Brassica Control 50 – no predicted protein in genome sequence | WISC (Brassica) | AV543465 | EC4H9 | ||
| 85 | F14C21.54 | Brassica Control 51 -similar to lipoxygenase 1 gene | WISC (Brassica) | AV544381 | EC5A1 | |
| 86 | Brassica Control 52 – EC5C4 | WISC (Brassica) | ||||
| 87 | Brassica Control 53 – EC5D1 | WISC (Brassica) | ||||
| 88 | T5K6_30 | Brassica Control 54 – MYB DNA-binding-like protein | WISC (Brassica) | AV523554 | EC5F3 | |
| 89 | At2g41250 | Brassica Control 55 – hypothetical protein | WISC (Brassica) | AI999138 | EC5F11 | |
| 90 | Arabidopsis genomic DNA 1 (10ug) | gDNA | ||||
| 91 | Arabidopsis genomic DNA 2 (5ug) | gDNA | ||||
| 92 | X65316 | Luciferase 1 (250ng/ul) | cDNA | |||
| 93 | X65316 | Luciferase 2 (100ng/ul) | cDNA | |||
| 94 | printing buffer (negative control) | printing buffer | ||||
| 95 | AF12601 | B-cell receptor protein (negative control) | cDNA | |||
| 96 | X07868 | Insulin-like growth factor (negative control) | cDNA |
* KDRI: Kazusa DNA Research Institute; ABRC: Arabidopsis Biological Resource Center; gDNA: genomic DNA amplified with indicated primer in parenthesis
WISC (Brassica): cDNA from University of Wisconsin
cDNA (TAMU): cDNA from Texas A&M University
** +: sequence verified; – : contain ambiguous sequence or more than one insert; blank : genomic fragments were not sequenced
