Gene List – Pilot Set III

| Pilot Set I | Pilot Set II | Pilot Set III |

No.	Gene ID	Gene Name	Source *	EST ID	Stock ID	Sequence Verification **
1	At1g56010	NAC1	cDNA (TAMU)			+
2	At3g49530	NAC2	cDNA (TAMU)			+
3	At1g53230	TCP3	cDNA (TAMU)			+
4	At1g53260	DAG	cDNA (TAMU)			+
5	At1g53250	Hypothetical protein3	cDNA (TAMU)			+
6	At1g53240	MNDMD	cDNA (TAMU)			+
7	At1g53210	Hypothetical protein2	cDNA (TAMU)			+
8	At1g53200	Hypothetical protein	cDNA (TAMU)			+
9	At1g53190	Ring finger protein	cDNA (TAMU)			+
10	At1g56360	Unknown gene-814	cDNA (TAMU)			+
11		Non coding RNA	cDNA (TAMU)			+
12	At1g79740	Transposase	cDNA (TAMU)			+
13	At5g58140	Kinase	cDNA (TAMU)			+
14	At4g36650	Transcription initiation factor	cDNA (TAMU)			+
15	At3g50670	U1 snRNP 70K protein	ABRC	AA394496	130B1XP	+
16	At3g53500	Splicing factor Arg/Ser rich 7	ABRC	T22516	105L5T7	+
17	At3g55200	Splicesomal protein SAP 130	ABRC	T42903	114O17T7	+
18	At3g60610	Splicing factor	ABRC	Z35043	ATTS3682	+
19	At3g61240	DEAD box RNA helicase RH12	ABRC	Z30728	ATTS2474	+
20	At3g61860	Arg/Ser-rich splicing factor RSP31	ABRC	T14106	47B7T7	+
21	At4g02430	Alternative splicing factor ASF	ABRC	Z25964	ATTS1241	+
22	At4g02840	Small nuclear riboprotein Sm-D1 protein	ABRC	T46269	139I4T7	+
23	At4g03430	Splicing factor	ABRC	AA394697	157A2XP	–
24	At4g16630	ATP dependent RNA helicase DRS	gDNA (hlcrDRS)	Z26774
25	At4g18460	RNA helicase Cdc28p	ABRC	H76018	193J17T7	+
26	At4g21660	Splicesome associated protein	ABRC	AA651260	178J20XP	+
27	At4g25500	Splicing factor At-SRp40	ABRC	H37668	185A17T7	+
28	At4g25630	Fibrillarin protein Sb21	gDNA (Fbllrn)	Z17495
29	At4g30220	snRNP Sm-F protein	ABRC	AA712720	193I11T7	+
30	At4g31580	RSZp22 splicing factor	ABRC	N65843	240D15T7	+
31	At4g36690	Splicing factor U2AF large chain	ABRC	N96704	G3A11T7	+
32	At4g34910	RNA helicase RH16	ABRC	T46809	143L17T7	+
33	At4g37120	Step II splicing factor	ABRC	H36844	179N2T7	+
34	At4g38780	Splicing factor Prp8	ABRC	T14115	47D7T7	+
35	At5g13010	Splicng factor ATP-dependent RNA helicase	ABRC	AA651273	179C6XP	+
36	At5g09690	MRS2 protein	ABRC	T22663	105N2T7	+
37	At4g38780	Splicing factor Prp8-exon1	gDNA (Prp8-ex1)
38	At4g38780	Splicing factor Prp8-exon2	gDNA (Prp8-ex2)
39	At4g38780	Splicing factor Prp8-exon3	gDNA (Prp8-ex3)
40	F17A9.17	Brassica Control 1 – UDP-glucose:sterol glucosyltransferase	WISC (Brassica)	T04809	WG1A4
41	At2g1911	Brassica Control 2 – putative heavy metal P-type ATPase	WISC (Brassica)	AV530075	WG1G10
42	F1N18.5	Brassica Control 3 – T31E10.2 photosystem II type I chlorophyl a/b binding protein	WISC (Brassica)	AV520935	WG1H5
43	F1N18.6	Brassica Control 4 – putative photosystem I type I chlorophyl a/b binding protein	WISC (Brassica)	AV521122	WG2C4
44	F3L17.13	Brassica Control 5 – putative protein	WISC (Brassica)	T75784	WG2D6
45	T5E21.11	Brassica Control 6 – valyl tRNA synthetase (valRS)	WISC (Brassica)	AV526903	WG2G11
46	At2g2082	Brassica Control 7 – unknown protein	WISC (Brassica)	AV554725	WG3G9
47	MBD2.17	Brassica Control 8 – COP8 homolog – note position verus 139!	WISC (Brassica)	AA394494	WG3G11
48	At2g3343	Brassica Control 9 – plastid protein	WISC (Brassica)	AV562631	WG4D7
49	At2g4607	Brassica Control 15 – putative mitogen – activated protein kinase	WISC (Brassica)	AV530854	WG8A9
50	At2g3683	Brassica Control 16 – putative aquaporin (tonoplast intrinsic protein gamma)	WISC (Brassica)	AV550938	TG2D6
51	F23N20.1	Brassica Control 17 – unknown protein	WISC (Brassica)	AV520594	TG5D9
52		Brassica Control 18 – EC2B3	WISC (Brassica)
53	MBD2.18	Brassica Control 19 – thioredoxin (clone GIF1)	WISC (Brassica)	AV554324	EC2C7
54	F17I14_1	Brassica Control 20 – microbody NAD-dependent malate dehydrogenase	WISC (Brassica)	AV525389	EC2D1
55	NWL2.17	Brassica Control 21 – predicted pseudogene	WISC (Brassica)	AV562296	EC2D8
56	At4g01150	Brassica Control 22 – predicted protein	WISC (Brassica)	AV441139	EC2D9
57		Brassica Control 23 – EC2D11	WISC (Brassica)
58	F4M19_50	Brassica Control 24 – putative protein	WISC (Brassica)	AV523700	EC2E12
59	F3C22_150	Brassica Control 25 – plastid division protein FtsZ mRNA	WISC (Brassica)	AV559837	EC2F1
60	MLD14.15	Brassica Control 26 – tyrosine phosphatase – like protein	WISC (Brassica)	AI994436	EC2H7
61		Brassica Control 27 – EC3B2	WISC (Brassica)
62		Brassica Control 28 – EC3B4	WISC (Brassica)
63	At4g05320	Brassica Control 29 – polyubiquitin (ubq10)	WISC (Brassica)	AV544113	EC3C8
64	At2g40000	Brassica Control 30 – putative nematode – resistance protein	WISC (Brassica)	AV555067	EC3D2
65		Brassica Control 31 – hit on BAC T8D3 end – not annotated	WISC (Brassica)	AV547452	EC3E10
66	F7G19.24	Brassica Control 32 – similarity to Picea histone H2A	WISC (Brassica)	T14194	EC3E12
67	F23M19.3	Brassica Control 33 – unknown	WISC (Brassica)	BE524433	EC3F1
68		Brassica Control 34 – EC3F3	WISC (Brassica)
69	F14D7.2	Brassica Control 35 – annexin (AnnAt1)	WISC (Brassica)	AV559022	EC3F4
70	At2g30410	Brassica Control 36 – putative TCP1 – chaperonin cofactor A	WISC (Brassica)	T14083	EC3F12
71	F2K13_150	Brassica Control 37 – quinone oxidoreductase – like protein	WISC (Brassica)	T21718	EC3G12
72	MUD21.18	Brassica Control 38 – pectinesterase like protein	WISC (Brassica)	AV537452	EC3H4
73	At2g32700	Brassica Control 39 – unknown protein	WISC (Brassica)	BE526178	EC4C5
74		Brassica Control 40 – EC4D9	WISC (Brassica)
75	At4g12600	Brassica Conrol 41 – Ribosomal protein L7Ae-like	WISC (Brassica)	T14197	EC4E7
76	F22O6_220	Brassica Control 42 – no predicted protein in genome sequence	WISC (Brassica)	AV565053	EC4E8
77	C7A10.500	Brassica Control 43 – putative protein	WISC (Brassica)	H76120	EC4F1
78	F9E10.20	Brassica Control 44 – unknown protein (T29M8.5) mRNA, complete cds	WISC (Brassica)	AV530446	EC4F10
79		Brassica Control 45 – no genome region and also weak EST hit	WISC (Brassica)	AV548183	EC4F11
80	MDA7.5	Brassica Control 46 – heat shock protein 90	WISC (Brassica)	AV439634	EC4G4
81	MLN21.1	Brassica Control 47 – transcription factor EREBP – like protein	WISC (Brassica)	AV553751	EC4G7
82	F28M20.10	Brassica Control 48 – putative protein similarity to DNA – binding protein ABF2, Avena	WISC (Brassica)	AV536499	EC4H3
83	F12A12.10	Brassica Control 49 – putative protein similarity to several oxidases, mainly gibberellin 20-oxidases	WISC (Brassica)	AV561112	EC4H7
84		Brassica Control 50 – no predicted protein in genome sequence	WISC (Brassica)	AV543465	EC4H9
85	F14C21.54	Brassica Control 51 -similar to lipoxygenase 1 gene	WISC (Brassica)	AV544381	EC5A1
86		Brassica Control 52 – EC5C4	WISC (Brassica)
87		Brassica Control 53 – EC5D1	WISC (Brassica)
88	T5K6_30	Brassica Control 54 – MYB DNA-binding-like protein	WISC (Brassica)	AV523554	EC5F3
89	At2g41250	Brassica Control 55 – hypothetical protein	WISC (Brassica)	AI999138	EC5F11
90		Arabidopsis genomic DNA 1 (10ug)	gDNA
91		Arabidopsis genomic DNA 2 (5ug)	gDNA
92	X65316	Luciferase 1 (250ng/ul)	cDNA
93	X65316	Luciferase 2 (100ng/ul)	cDNA
94		printing buffer (negative control)	printing buffer
95	AF12601	B-cell receptor protein (negative control)	cDNA
96	X07868	Insulin-like growth factor (negative control)	cDNA

* KDRI: Kazusa DNA Research Institute; ABRC: Arabidopsis Biological Resource Center; gDNA: genomic DNA amplified with indicated primer in parenthesis
WISC (Brassica): cDNA from University of Wisconsin
cDNA (TAMU): cDNA from Texas A&M University
** +: sequence verified; – : contain ambiguous sequence or more than one insert; blank : genomic fragments were not sequenced

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