The Proteomics Facility is housed in dedicated instrumentation laboratories located in MBB 1.420 and PHR 5.220.
The following instruments are included in the facility with installation date in parantheses.
Two Thermo Orbitrap Fusions (2017), one with ETD fragmentation (2015) coupled to Dionex Ultimate 3000 RSLCnano Nanoflow UPLCs.
MS and CID, HCD or ETD MSn analysis with electrospray ionization. Protein identification from gel bands, gel sections, and solution mixtures. Proteome Discoverer Sequest HT, MS-Amanda, X!Tandem, and MASCOT database search engines, Scaffold data visualization, validation, quantitation with spectral counting or stable isotope labeling. Post-translational modification site determination. Byonic post-translational modification and crosslinking analysis, XlinkX crosslinking determination.
Intavis DigestPro MSi (2016)
The Intavis DigestPro MSi is an automated robotic platform for performing enzymatic digests of protein samples submitted to the lab. Along with fully automated in-gel and in-solution digest capabilities, the DigestPro can also perform automated ziptipping procedures, which has higher reproducibility in comparison to manual ziptip procedures.
AB Voyager-DE PRO MALDI-TOF (2001)
MALDI-MS is suitable for many types of biomolecules, including proteins, peptides, lipids, polymers, drugs and chemicals. The instrument has linear and reflector detectors. Self-service training available for Voyager.
More information at: MALDI-MS
Dionex Ultimate 3000 UPLC (2015)
Biorad BioLogic DuoFlow FPLC (2 systems-room temp. and 4 C) (2007)
Dionex LC Packings nanoLC (2004)
Self-service FPLC for high resolution purification of proteins and peptides when recovery of activity is important.
Flow rates ul/min-ml/min.
More information at: LC Systems
Protein/Peptide Concentration Measurement
EMD Millipore Direct Detect IR Spectrometer (2012)
Uses FTIR to quantitate proteins or peptides in solution without interference from reagents (most detergents etc)
Training and space provided for self-service gel electrophoresis