Jon M. Huibregtse, Ph.D.
Department of Molecular Biosciences, University of Texas at Austin
2500 Speedway, Austin, TX 78712
Phone: 512-232-7700, email: huibregtse@jmh1960
Education:
B. S., 1983, Biochemistry, University of Michigan.
Ph.D., 1989, Department of Biological Chemistry, University of Michigan. Thesis: Protein-DNA interactions at tRNA gene promoter regions and at an RNA polymerase II upstream activation sequence in yeast nuclei. PI: Dr. David Engelke.
Research and Academic Positions:
Postdoctoral fellow, Laboratory of Tumor Virus Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, 1989 to 1993. PI: Dr. Peter Howley
Instructor, Department of Pathology, Harvard Medical School, Boston, MA, 1993 to 1995.
Assistant Professor, Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ, 1995 to 2000.
Associate Professor, Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, 2000 to 2007.
Professor, Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, 2007 to present.
Professor and Interim Chair, Department of Molecular Biosciences, University of Texas at Austin, Sept., 2014 – Jan., 2016.
Director, Institute for Cellular and Molecular Biology, University of Texas at Austin, Sept., 2016 – Aug. 2020. Benjamin Clayton Centennial Professor of Biochemistry, Department of Molecular Biosciences, 2020 – present.
Fellowships and Awards:
National Institutes of Health Genetics Pre-Doctoral Fellowship (1983-1987).
University of Michigan Cancer Research Institute Fellowship (1987-1988).
National Institutes of Health Intramural Research Training Award, Nov., 1989 – Oct., 1991.
American Cancer Society Postdoctoral Fellowship, Nov., 1991 – Oct., 1993.
College of Natural Sciences Teaching Excellence Award, 2004.
Scientific Review Panels:
Ad hoc reviewer, NIH Study Section, Special Emphasis Panel on Bioterrorism and Emerging Infectious Diseases, Mar. 2003
Ad hoc reviewer, NIH Biochemistry Study Section, June, 2003
Ad hoc reviewer, NIH Study Section, Special Emphasis Panel on Bioterrorism and Emerging Infectious Diseases, Oct. 2003
National Cancer Institute, Program Project (PO1) Review Panel, April, 2006.
National Cancer Institute, Program Project (PO1) Review Panel, June, 2008.
Ad hoc reviewer, NIH Membrane Proteins and Protein Processing (MBPP) Study Section, Oct. 2008
Ad hoc reviewer, NIH Membrane Proteins and Protein Processing (MBPP) Study Section, May, 2009
Permanent member, NIH Membrane Proteins and Protein Processing (MBPP) Study Section, 2011-2016
Conference Organization:
FASEB Summer Research Conference on Ubiquitin and Cellular Regulation, July, 2010, Saxtons River, VT. Co-Organizer.
FASEB Summer Research Conference on Ubiquitin and Cellular Regulation, July, 2012, Saxtons River, VT. Organizer.
Conference Presentations:
The 6th p53 Workshop, Tiberias, Israel, Nov., 1992
p53 in Growth Control and Neoplasia, Zurich, Switzerland, Mar., 1993
Basic and Applied Cancer Research International Symposium on Human Papillomaviruses, Sao Paulo, Brazil, Apr., 1993
American Association for Cancer Research Annual Meeting, Orlando, Florida, May, 1993
Gordon Conference, Animal Cells and Viruses, Tilton, New Hampshire, June, 1993
The 7th p53 Workshop, Toronto, Canada, June, 1994
The Targets and Regulation of the Ubiquitin System, Banbury Center, Cold Spring Harbor Laboratory, Feb., 1995
Ubiquitin and Protein Degradation, FASEB Summer Research Conference, June, 1997
3rd Cleveland Virology Symposium, Case Western Reserve University, Oct., 1997
Biology of Proteolysis, Cold Spring Harbor Laboratory, May, 1999.
Ubiquitin and Protein Degradation, FASEB Summer Research Conference, Aug., 1999
DNA Tumor Viruses, University of Wisconsin, Madison, July, 2000.
Ubiquitin and Protein Degradation, FASEB Summer Research Conference, July, 2001
Cold Spring Harbor Ubiquitin Family meeting, Apr., 2003
Ubiquitination and Cellular Regulation, FASEB Summer Research Conference, June, 2004
Cold Spring Harbor Ubiquitin Family meeting, Apr., 2005
Ubiquitin and Cellular Regulation, FASEB Summer Research Conference, July, 2006.
Cold Spring Harbor Ubiquitin Family meet, Apr., 2007
Ubiquitin and Cellular Regulation, FASEB Summer Research Conference, July, 2008.
Angelman Syndrome Foundation Scientific Symposium, Boston, MA, Aug. 2008.
Cold Spring Harbor Ubiquitin Family meeting, Apr., 2009.
INSERM Workshop on “Ubiquitin, ubiquitin like-proteins and proteasomes: functions and dysfunctions,” Saint-Raphael, France, June, 2009.
Fifth International Conference on SUMO, Ubiquitin, Ubl Proteins: Implications for Human Diseases. M. D. Anderson Cancer Center, Houston, TX, Feb., 2010.
RUBICON conference on Ubiquitin Chain Formation, Paris, France, April, 2010.
Banbury Conference on Ubiquitin Signaling, Cold Spring Harbor Laboratory, NY, Nov., 2010.
EMBO Ubiquitin Conference, Dubrovnik, Croatia, Nov., 2011
Keystone Conference on Ubiquitin Signaling, Whistler, British Columbia, Mar., 2012
FASEB Summer Research Conference, Ubiquitin and Cellular Regulation, Saxtons River, VT, July 2012 (Conference organizer)
ZOMES VII, Ubiquitin-Like Proteins, Munich, Germany, Sept., 2012
Cold Spring Harbor “Ubiquitin Family”, Cold Spring Harbor Laboratory, NY, May, 2013
Angelman Syndrome Scientific Symposium, Orlando, FL, July, 2013
FASEB Summer Research Conference, Ubiquitin and Cellular Regulation, Saxtons River, VT, June 2014
EMBO Conference on Ubiquitin and Ubiquitin-Like Modifiers, Cavtat, Croatia, Sept. 2015
FASEB Summer Research Conference, Ubiquitin and Cellular Regulation, Big Sky, Montana, June 2016
Cold Spring Harbor “Ubiquitin Family”, Cold Spring Harbor Laboratory, NY, April, 2017
Keystone Conference on Ubiquitin Signaling, South Lake Tahoe, CA, Jan., 2018.
Cold Spring Harbor Conference of Ubiquitin, Autophagy and Disease. Speaker, Virtual Conference. Feb., 2021
The ISG15 System in Molecular in Molecular Function and Disease Mechanisms. EMBO Workshop, June 2022, Berlin, Germany.
Invited Seminars:
University of Colorado Health Science Center, Denver, CO, Dec., 1992
Baylor College of Medicine, Houston, TX, Feb., 1993
St. Jude’s Children’s Hospital, Memphis, TN, Feb., 1993
University of California at Santa Barbara, Santa Barbara, CA, Feb., 1995.
Ligand Pharmaceuticals, San Diego, CA, Feb., 1995.
University of California at Irvine, Irvine, CA, Feb., 1995.
Stanford University, Palo Alto, CA, Apr., 1995.
University of North Carolina, Chapel Hill, NC, Apr., 1995.
Mount Sinai Medical Center, New York, NY, Oct., 1995.
Wayne State University, Detroit, MI, Oct., 1996.
Bristol Meyers-Squibb, Princeton, NJ, Nov., 1996.
University of Michigan Medical School, May, 1997.
Fox Chase Cancer Center, Philadelphia, PA, Oct., 1997
Hershey Medical Center, Hershey, PA, Nov 1997
Queens College, Queens, NY, Dec., 1997.
Axcell Corporation, Princeton, NJ, Jan., 1998.
Georgia State University, Atlanta, GA, Jan., 1998 .
Uniformed Services University of the Health Sciences, Bethesda, MD, Feb., 1998
Schering-Plough Research Institute, Kenilworth, NJ, March, 1999
University of Texas, Austin, TX, April, 1999.
University of Medicine and Dentistry of New Jersey, Newark, NJ, Nov. 9, 1999
University of Michigan, Ann Arbor, MI, Oct. 30, 2001
University of North Carolina, Chapel Hill, NC, Oct., 2003
Texas A&M University, College Station, TX, Nov., 2003
University of Texas San Antonio Health Science Center, Apr., 2005
Rutgers University, Piscataway, NJ, Oct., 2005
St. Louis University, St. Louis, MO, Nov., 2005
University of Texas San Antonio Health Science Center, Oct., 2007
Purdue University, Nov., 2007.
University of Coimbra, Portugal, Jan. 2008 (part of a one week course on the ubiquitin system).
Hospital for Sick Kids, University of Toronto, Nov. 2008
Baylor College of Medicine, Houston, TX, Jan., 2011
Dalhousie University, Oct., 2012
University of South Florida, Nov., 2012
Harvard Medical School, Department of Microbiology and Immunobiology, Dec., 2012
University of Michigan, Department of Cellular, Molecular, and Developmental Biology, Sept., 2013
Wayne State University, Karmanos Cancer Center, Detroit, MI, Jan., 2014
Mount Sinai Cancer Center, New York, NY, May, 2014
University of Washington, Department of Biochemistry, Seattle, WA, Dec., 2014
Vanderbilt University, Dept. of Cell and Developmental Biology, Nashville, TN, Oct., 2015
Cornell University, Ithaca, NY., Feb., 2017
University of Pennsylvania, Children’s Hospital of Philadelphia, Phil., PA, Mar., 2018
Vanderbilt University, Nov., 2021
Distinguished Alumni Lecture, University of Michigan, Department of Biological Chemistry, Apr., 2022.
Publications:
Huibregtse, J. M., and Engelke, D. R. 1986. Direct identification of small sequence changes in chromosomal DNA. Gene 44:151-158.
Huibregtse, J. M., Evans, C. F., and Engelke, D. R. 1987. Comparison of tRNA gene transcription complexes formed in vitro and in nuclei. Mol. Cell. Biol. 7:3212-3220.
Huibregtse, J. M., and Engelke, D. R. 1989. Genomic footprinting of a yeast tRNA gene reveals stable complexes over the 5′-flanking region. Mol. Cell. Biol. 9:3244-3252.
Huibregtse, J. M., Engelke, D. R., and Thiele, D. J. 1989. Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences. Proc. Natl. Acad. Sci. U.S.A. 86:65-69.
Scheffner, M., Werness, B. A., Huibregtse, J. M., Levine, A. S., and Howley, P. M. 1990. The E6 oncoprotein encoded by human papillomavirus 16 and 18 promotes the degradation of p53. Cell 63:1129-1136.
Huibregtse, J. M., and Engelke, D. R. 1991. Direct sequence and footprint analysis of yeast DNA by primer extension. Meth. Enzym. 194:550-562.
Huibregtse, J. M., Scheffner, M., and Howley, P. M. 1991. A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18. The EMBO J. 10:4129-4135.
Hull, M. W., Thomas, G., Huibregtse, J. M., and Engelke, D. R. 1991. Protein-DNA interactions in vivo – examining genes in Saccharomyces cerevisiae and Drosophila melanogaster by chromatin footprinting. Methods Enzym. 194:550-562.
Howley, P. M., Scheffner, M., Huibregtse, J. M., and Münger, K. 1991. Oncoproteins encoded by the cancer-associated human papillomaviruses target the products of the retinoblastoma and p53 tumor suppressor genes. Cold Spring Harbor Symp. on Quant. Biol. LVI:149-155.
Howley, P. M., Munger, K., Romanczuk, Scheffner, M., and Huibregtse, J. M. 1991. Cellular targets of the oncoproteins encoded by the cancer associated human papillomaviruses. Princess Takamatsu Symp. 22:239-248.
Scheffner, M., Münger, K., Huibregtse, J. M., and Howley, P. M. 1992. Targeted degradation of the retinoblastoma protein by human papillomavirus E7-E6 fusion proteins. The EMBO J. 11:2425-2431.
Meitz, J. A., Unger, T., Huibregtse, J. M., and Howley, P. M. 1992. The transcriptional transactivation function of wild-type p53 is inhibited by SV40 large T antigen and by HPV16 E6 oncoprotein. EMBO J. 11:5013-5020.
Scheffner, M., Takahashi, T., Huibregtse, J. M., Minna, J. D., and Howley, P. M. 1992. Interaction of the HPV-16 E6 oncoprotein with wild-type and mutant human p53 proteins. J. Virol. 66:5100-5105.
Munger, K., Scheffner, M., Huibregtse, J. M., and Howley, P. M. 1992. Interaction of HPV E6 and E7 oncoproteins with tumor suppressor gene products. Cancer Surveys. 12:197-217.
Huibregtse, J. M., Good, P.D., Marczynski, G. T., Jaehning, J. A., and Engelke, D. R. 1993. Gal4 protein binding is required but is not sufficient for derepression and induction of GAL2 expression. J. Biol. Chem. 268:22219-22222.
Huibregtse, J. M., Scheffner, M., and Howley, P. M. 1993. Cloning and expression of the cDNA for E6-AP: a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53. Mol. Cell. Biol. 13:775-784.
Huibregtse, J. M., Scheffner, M., and Howley, P. M. 1993. Localization of the E6-AP regions that direct HPV E6 binding, association with p53, and ubiquitination of associated proteins. Mol. Cell. Biol. 13:4918-4927.
Scheffner, M., Huibregtse, J. M., Vierstra, R. D., and Howley, P. M. 1993. The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. Cell 75:494-505.
Huibregtse, J. M., and Scheffner, M. 1994. Mechanisms of tumor suppressor protein inactivation by the human papillomavirus E6 and E7 oncoproteins. Semin. in Virol. 5:502-512.
Scheffner, M., Huibregtse, J. M., and Howley, P. M. 1994. Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Proc. Natl. Acad. Sci. USA. 91:8797-8801.
Scheffner, M., Romanczuk, H., Munger, K., Huibregtse, J. M., Mietz, J. A., and Howley, P. M. 1994. Functions of human papillomavirus proteins. Curr. Top. in Micro. Immun. 186:83-99.
Huibregtse, J. M., Scheffner, M., and Howley, P. M. 1994. E6-AP directs the HPV E6-dependent inactivation of p53 and represents a family of structurally and functionally related proteins. Cold Spring Harbor Symp. Quant. Biol., LIX:237-245.
Scheffner, M., Nuber, U., and Huibregtse, J. M. 1995. Protein ubiquitination involving an E1-E2-E3 enzyme ubiquitin thioester cascade. Nature 373:81-83.
Huibregtse, J. M., Scheffner, M., Beaudenon, S., and Howley, P. M. 1995. A family of proteins structurally and functionally related to the E6-AP ubiquitin protein ligase. Proc. Natl. Acad. Sci. U.S.A. 92:2563-2567.
Maki, C. G., Huibregtse, J. M., and Howley, P. M. 1996. In vivo ubiquitination and proteasome-mediated degradation of p53. Cancer Research 56:2649-2654.
Yamamoto, Y., Huibregtse, J. M., and Howley, P. M. 1997. The human E6-AP gene (UBE3A) encodes three potential protein isoforms generated by differential splicing. Genomics, 41:263-266.
Huibregtse, J. M., and Beaudenon, S. L. 1997. Mechanism of HPV E6 Proteins in Cellular Transformation. Seminars in Cancer Biology. 7:317-326.
Huibregtse, J. M., Yang, J., and Beaudenon, S. L. 1997. The large subunit of RNA polymerase II is a substrate of the Rsp5 ubiquitin-protein ligase. Proc. Natl. Acad. Sci, USA 94:3656-3661.
Talis, A. L., Huibregtse, J. M., and Howley, P. M. 1998. The role of E6-AP in regulating p53 protein levels in vivo. J. Biol. Chem. 273:6439-6445.
Wang, G., Yang, J., and Huibregtse, J. M. 1999. Functional domains of the Rsp5 ubiquitin-protein ligase. Mol. Cell. Biol. 19:342-352.
Beaudenon, S. L., Huacani, M. R., McDonnell, D. P., and Huibregtse, J. M. 1999. The Rsp5 ubiquitin-protein ligase mediates DNA damage-induced degradation of the large subunit of RNA polymerase II in Saccharomyces cerevisiae. Mol. Cell. Biol. 19:6972-6979.
Huang, L., Kinnucan, E., Wang G., Beaudenon, S., Howley, P. M., Huibregtse, J. M., and Pavletich, N. P. 1999. Structure of an E6AP-UbcH7 complex: insights into the ubiquitin ligase/conjugating enzyme cascade. Science286:1321-1326.
Kao, W., Beaudenon, S., Talis, A., Huibregtse, J. M., and Howley, P. M. 2000. Human papillomavirus type 16 E6 induces self-ubiquitination of the E6AP ubiquitin-protein ligase. J. Virol. 74:6408-6417.
Harty, R. N., Brown, M. E., Wang, G., Huibregtse, J., and Hayes, F. P. 2000. A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: Implications for filovirus budding. Proc. Natl. Acad. Sci. U. S. A. 97:13871-13876.
Bilder, D., Birnbaum, D., Borg, J. P., Bryant, P., Huibregtse, J., Jansen, E., Kennedy, M. B., Labouesse, M., Legouis, R., Mechler, B., Perrimon, N., Petit, M., Sinha, P. 2000. Collective nomenclature for LAP proteins. Nat. Cell Biol. 7:E114.
Nakagawa, S., and Huibregtse, J. M. 2000. Human Scribble (Vartul) is targeted for ubiquitin-mediated degradation by the high-risk papllimavirus E6 proteins and the E6AP ubiquitin-protein ligase. Mol. Cell. Biol. 20:8244-8253.
Wang, G., McCaffrey, J. M., Wendland, B., Dupré, S., Haguenauer-Tsapis, R., and Huibregtse, J. M. 2001. Localization of the Rsp5p ubiquitin-protein ligase at multiple sites within the endocytic pathway. Mol. Cell. Biol. 21:3564-3575.
Hamilton, M., Tcherepanova, I., Huibregtse, J. M., and McDonnell, D. P. 2001. Nuclear import/export of hRPF1/Nedd4 regulates the ubiquitin-dependent degradation of its nuclear substrates. J. Biol. Chem. 276:26324-26331.
Harty, R. N., Brown, M. E., McGettigan, J. P., Wang, G., Jayakar, H. R., Huibregtse, J. M., Whitt, M. A., and Schnell. M. J. 2001. Rhabdoviruses and the cellular ubiquitin-proteasome system: a budding interaction. J. Virol. 75: 10623-10629.
Gao, O., Kumar, A., Singh, L., Huibregtse, J. M., Beaudenon, S., Srinivasan, S., Wazer, D. E., Band, H., and Band, V. 2002. Human papillomavirus E6-induced degradation of E6TP1 is mediated by E6AP ubiquitin ligase. Cancer Res. 62:3315-3321.
Nakagawa S., Yano, T., Nakagawa, K., Takizawa, S., Suzuki, Y., Yasugi, T., Huibregtse, J. M., Taketani, Y. 2004. Analysis of the expression and localisation of a LAP protein, human scribble, in the normal and neoplastic epithelium of uterine cervix. Br. J. Cancer. 90:194-199.
Salvat, C., Wang, G., Dastur, A., Lyon, N., and Huibregtse, J. M. 2004. The -4 phenylalanine is required for substrate ubiquitination catalyzed by HECT ubiquitin ligases. J. Biol. Chem. 279:18935-18943
Zhao, C., Beaudenon, S., Kelley, M. L., Waddell, M. B., Yuan, W., Schulman, B. A., Huibregtse, J. M., and Krug, R. M. 2004. The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an interferon a/b-induced ubiquitin-like protein. Proc. Natl. Acad. Sci. USA. 101:7578-7582.
Shcherbik, N., Kee, Y., Lyon, N., Huibregtse, J. M., and Haines, D. S. 2004. A Single PXY Motif Located within the Carboxyl Terminus of Spt23p and Mga2p Mediates a Physical and Functional Interaction with Ubiquitin Ligase Rsp5p. J. Biol. Chem. 279:53892-53898.
Liu, X., Yuan, H., Fu, B., Disbrow, G. L., Apolinario, T., Tomaic, V., Kelley, M. L, Baker, C. C., Huibregtse, J., and Schlegel, R. 2005. The E6-AP ubiquitin ligase is required for transactivation of the hTERT promoter by the human papillomavirus E6 oncoprotein. J. Biol. Chem. 280:10807-10816.
Beaudenon, S., and Huibregtse, J. M. 2005. High level expression and purification of recombinant E1 enzyme. Methods in Enzymology, 398:3-8.
Beaudenen, S., Dastur, A., and Huibregtse, J. M. 2005. Expression and assay of HECT domain ligases. Methods in Enzymology, 398:112-124.
Kelley, M. L., Keiger, K. E., Lee, C. J., and Huibregtse, J. M. 2005. The global transcriptional effects of the HPV E6 protein in cervical carcinoma cell lines are mediated by the E6AP ubiquitin ligase. J. Virol. 79:3737-3747.
Huibregtse, J. M. 2005. UPS Shipping and Handling. Cell 120:2-4.
Zhao, C., Denison, C., Huibregtse, J. M., Gygi, S., and Krug, R. M. 2005. Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways. Proc. Natl. Acad. Sci. USA. 102:10200-10205.
Kee, Y., Lyon, N., and Huibregtse, J. M. 2005. The Ubp2 deubiquitinating enzyme associates with and antagonizes the Rsp5 ubiquitin ligase. EMBO J. 24:2414-2424.
Matsumoto, Y., Nakagawa, S., Yano, T., Takizawa, S., Nagasaka, K., Nakagawa, K., Minaguchi, T., Wada, O., Ooishi, H., Matsumoto, K., Yasugi, T., Kanda, T., Huibregtse, J. M., and Taketani, Y. 2006. Involvement of a cellular ubiquitin-protein ligase E6AP in the ubiquitin-mediated degradation of extensive substrates of high-risk human papillomavirus E6. J. Med. Micro. 78:501-507.
Takizawa, S., Nagasaka, K., Nakagawa, S., Yano, T., Nakagawa, K., Yasugi, T., Takeuchi, T., Kanda, T., Huibregtse, J. M., Akiyama, T., and Taketani, Y. 2006. Human scribble, a novel tumor suppressor identified as a target of high-risk HPV E6 for ubiquitin-mediated degradation, interacts with adenomatous polyposis coli. Genes Cells. 11:453-464.
Dastur, A., Beaudenon, S., Kelley, M., Krug, R. M., and Huibregtse, J. M. 2006. Herc5, an interferon-induced HECT E3 enzyme, is required for conjugation of human ISG15. J. Biol. Chem. 281:4334-4338.
Kee, Y., Munoz, W., Lyon, N., and Huibregtse, J. M. 2006. Ubp2 modulates Rsp5-dependent K63-linked polyubiquitin conjugates in Saccharomyces cerevisiae J. Biol. Chem. 281:36724-31.
Ren, J., Kee, Y., Huibregtse, J. M., and Piper, R. C. 2007. Hse1, a component of the yeast Hrs-STAM ubiquitin sorting complex, binds both ubiquitin peptidases and ligases to control sorting efficiency into multivesicular bodies. Mol. Biol. Cell, 18(1):324-35.
Kee, Y., and Huibregtse, J. M. 2007. Regulation of catalytic activities of HECT ubiquitin ligases. Biochem. Biophys. Res. Comm. 354:329-33.
Munakata, T., Liang, Y., Kim, S., McGivern, D., Huibregtse, J., Nomoto, A., and Lemon, S. Hepatitis C virus indiced, E6-AP-dependent degradation of the retinoblastoma protein. 2007. PloS Pathogens 3:1335-1347.
Diao, J., Zhang, Y., Huibregtse, J. M., Zhou, D., and Chen, J. 2008. Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Nat. Struct. Mol. Biol. 15:65-70.
Beaudenon, S., and Huibregtse, J. M. 2008. The HPV E6 protein and E6AP in cervical cancer. BMC Biochemistry. 9Suppl:1-54.
Durfee, L. A., Kelley, M. L., and Huibregtse, J. M. 2008. The basis for selective E1-E2 interactions in the ISG15 system. J. Biol. Chem. 283:23895-902.
Kim, HC and Huibregtse, J. M. 2009. Polyubiquitination by HECT E3s and the Determinants of Chain Type Specificity. Mol. Cell. Biol. 29:3307-3318.
Durfee, L. A., and Huibregtse, J. M. 2010. Identification and validation of ISG15 target proteins. In Conjugation and Deconjugation of Ubiquitin Family Modifiers, edited by M. Groettrup. Landes Bioscience.
Durfee, L. A., Lyon, N., Seo, K., and Huibregtse, J. M. 2010. The ISG15 conjugation system broadly targets newly synthesized proteins: implications for the anti-viral function of ISG15. Mol. Cell 38:722-732.
Weiss, E. R., Popova, E., Yamanaka, H., Kim, H. C., Huibregtse, J. M., Göttlinger, H. 2010. Rescue of HIV-1 release by targeting widely divergent NEDD4-type ubiquitin ligases and isolated catalytic HECT domains to Gag. PLoS Pathog. 6:e1001107.
Kim, H. C., Steffen, A., Chen, J., and Huibregtse, J. M. 2011. Structure and Function of a ubiquitin binding site within the catalytic domain of a HECT ubiquitin ligase. EMBO Reports, 12:334-341.
Bogunovic, D., Byun, M., Abhyankar, A., Durfee, L. A., Sanal, O., Mansouri, D., Grant, A. V., Adimi, P., Mansouri, N., Okada, S., Bryant, V., Kong, S.-F., Kreins, A., Boisson, B., Zadeh, S. K., Darazam, I. A., Schoggins, J. W., Rice, C. C., Al-Muhsen, S., Bustamante, J., Huibregtse, J. M., Abel, L., Boisson-Dupuis, S., and Casanova, J.-L. 2012. Impaired IFN-g immunity and mycobacterial disease in humans with inherited ISG15 deficiency. Science 337:1684-88.
Feng, W., Durfee, L. A., and Huibregtse, J. M. 2013. Cotranslational ubiquitination mediates the degradation of newly synthesized proteins. Mol. Cell 50:368-378.
Huibregtse, J. M, and Rohde, J. R. 2014. Hell’s BELs: Bacterial E3 Ligases that exploit the eukaryotic ubiquitin machinery. PLoS Pathogens 10(8):e1004255.
Wang, F., Canadeo, L., and Huibregtse, J. M. 2015. Ubiquitination of newly synthesized proteins at the ribosome. Biochemie 114:127-133.
O’Connor, H., Lyon, N., Leung, J. W., Agarwal, P., Swaim, C., Miller, K. M., and Huibregtse, J. M. 2015. Ubiquitin-Activated Interaction Traps (UBAITs) identify E3 ligase binding partners. EMBO Reports 16:1699-1712.
Huibregtse, J. M., and Matouschek, A. 2016. Ramping up degradation for proliferation. Nature Cell Biol. 18:141-142.
Canadeo, L. A., and Huibregtse, J. M. 2016. A billion ubiquitin variants to probe and modulate the UPS. Mol. Cell 62:2-4.
O’Connor, H. F., and Huibregtse, J. M. 2017. Enzyme-substrate relationships in the ubiquitin system: approaches for identifying substrates of ubiquitin ligases. Cell Mol Life Sci. 74:3363-3375.
Swaim, C. D., Scott, A. F., Canadeo, L. A., and Huibregtse, J. M. 2017. Extracellular ISG15 signals cytokine secretion through the LFA-1 integrin receptor. Mol. Cell 68:581-590.
O’Connor, H. F., Swaim, C. D., Canadeo, L. A., and Huibregtse, J. M. 2018. Ubiquitin-Activated Interaction Traps (UBAITs): Tools for capturing protein-protein interactions. In The Ubiquitin Proteasome System: Methods and Protocols. Methods in Mol. Biol., Springer, 1844:85-100.
Swaim, C. D., Canadeo, L. A., and Huibregtse, J. M. Approaches for investigating the extracellular signaling function of ISG15. Methods in Enzymology 618:211-227.
Tomita, T., Huibregtse, J. M., and Matouschek, A. 2020. A masked initiation region in retinoblastoma protein regulates its proteasomal degradation. Nature Commun. 11:2019.
Ryu, W. W., Stewart, R., Pectol, C., Ender, N., Wimalarathne, O., Lee, J.-H., Zanini, C.P., Harvey, A., Huibregtse, J. M., Mueller, P., And Paull, T. T. 2020. Proteome-wide identification of HSP70/HSC70 chaperone clients in human cells. PLoS Biology 18:e3000606.
Swaim, C. D., Canadeo, L. A., Monte, K. J., Khanna, S., Lenschow, D. J., and Huibregtse, J. M. 2020. Modulation of extracellular ISG15 signaling by pathogens and viral effector proteins. Cell Reports 31:107772.
Swaim CD, Perng YC, Zhao X, Canadeo LA, Harastani HH, Darling TL, Boon ACM, Lenschow DJ, and Huibregtse JM. 2021. 6-Thioguanine blocks SARS-CoV-2 replication by inhibition of PLpro. iScience doi: 10.1016/j.isci.2021.103213.
Service, University of Texas:
University Intellectual Property Committee, 2002-2005
Member, Faculty Search Committee, Section of Molecular Genetics and Microbiology, 2002
CMB Graduate Program Admissions Committee, 2004
College of Natural Sciences Course and Curriculum Committee, 2003-2005
Graduate Advisor, Microbiology Graduate Program, 2005-2007
Graduate Advisor, Cellular and Molecular Biology Program, 2007-present
Organizer, Annual ICMB retreat, 2002 – present.
Co-chair, ICMB faculty search committee, 2006, 2007, 2008, 2010
Member, MCDB Cell Biology faculty search committee, 2012
Member, Health Science Honors Program Steering Committee, 2012-present
Interim Chair, Department of Molecular Biosciences, 2014-2016
Director, Institute for Cellular and Molecular Biology, University of Texas at Austin, 2016- 2020
Co-chair, Department of Molecular Biosciences Faculty Search Committee, 2019
Department of Molecular Biosciences, Chair of the Research Infrastructure Committee, 2021-present.
Postdoctoral fellow trainees:
Catherine Salvat (1998-2002)
Shunsuke Nakagawa (1998-2001)
Melissa Kelley (2001-2005)
Sylvie Beaudenon (Research Scientist 2000-2005)
Larissa Durfee (2010-present)
Hazel O’Connor (2012-2016)
Hugo Miranda (2015-2017)
Jessica Perez (2017-present)
Graduate Student trainees:
Guangli Wang, Ph.D., Rutgers University, 2000.
Steve Jung, M.S., Rutgers University, 2000.
Younghoon Kee (UT Cellular and Molecular Biology Program)
Anahita Dastur (UT Cellular and Molecular Biology Program)
Larissa Durfee (UT Cellular and Molecular Biology Program)
Hyung Cheol Kim (UT Microbiology Graduate Program)
Kyungwoon Seo (UT CMB Graduate Program)
Feng Wang (UT Microbiology Graduate Program)
Aaron Charlson (UT Microbiology Program)
Amanda Vaughn (UT CMB Graduate Program)
Nicholas Maskalenko (UT CMB Graduate Program)
Caleb Swaim (UT Microbiology Graduate Program)
Xu Zhao (UT CMB Graduate Program)
Cecelia Fitzgerald-Cook (UT CMB Graduate Program)
Undergraduate Trainees (University of Texas at Austin):
Amanda Joyner (2001-2002)
Sebastian Paige (2000-2001)
Christina Stuart (2004)
Ryan Nielen (2004)
Juan Carlos Martinez (2005)
Tracy Chang (2004-2005)
William Munoz (2006-8)
Jennifer Nolsch (2007-2009)
Emily Camp (2009-2010)
Michelle Faria (2011)
Alessandra Rossi (2011-2013)
Jerry So (2013-2015)
Avery Wolfe (2015-)
Samantha Finkenstaedt (2015-2016)
Hunter Flores (2016-2018)
Ariella Scott (2016-2018)
Steven Kim (2017-2019)
Rianna Godula (2021-present)
Catherine Chan (2021-present)
Courses Taught (University of Texas at Austin):
Bio 336/391M, Tumor Biology: 2002 – 2022
Bio 393 (Microbiology Journal Club): Spring 2001, 2004
Bio 393 – Microbiology Student Seminar Series, Fall 2008, 2009, 2010