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Lambowitz Lab
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  • Research Summary
    • (A) Mobile Group II Intron, Evolutionary Ancestors of Retroviruses, Retrotransposons, and Spliceosomal Introns in Eukaryotes.
    • (B) Group II Intron Reverse Transcriptases
    • (C) Reverse Transcriptases Associated with CRISPR-Cas Systems and Other Bacterial and Organellar Reverse Transcriptases
    • (D) Thermostable Group II Intron Reverse Transcriptases (TGIRTs) and Their Applications in High Throughput RNA Sequencing (RNA-Seq).
    • (E) Analysis of Extracellular RNAs, RNA Diagnostics, and Liquid Biopsy and tRNA fragments
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    • Principal Investigator
    • Assistant to Dr. Lambowitz
    • Postdoctoral Associates
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Lambowitz Lab

Department of Molecular Biosciences

100 East 24th St., Stop A5000
Austin, TX 78712 (email)

2013

December 13, 2024, Filed Under: Publications

Enhanced group II intron retrohoming in magnesium-deficient Escherichia coli via selection of mutations in the ribozyme core.

Citation:

Truong DM, Sidote DJ, Russell R, Lambowitz AM. Enhanced group II intron retrohoming in magnesium-deficient Escherichia coli via selection of mutations in the ribozyme core. Proc Natl Acad Sci U S A [Internet]. 110 (40) :E3800-9.

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Last updated on 02/12/2015

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December 13, 2024, Filed Under: Publications

Generalized bacterial genome editing using mobile group II introns and Cre-lox.

Citation:

Enyeart PJ, Chirieleison SM, Dao MN, Perutka J, Quandt EM, Yao J, Whitt JT, Keatinge-Clay AT, Lambowitz AM, Ellington AD. Generalized bacterial genome editing using mobile group II introns and Cre-lox. Mol Syst Biol [Internet]. 9 :685.

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Last updated on 02/12/2015

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December 13, 2024, Filed Under: Publications

Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming.

Citation:

Yao J, Truong DM, Lambowitz AM. Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming. PLoS Genet [Internet]. 9 (4) :e1003469.

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Last updated on 02/12/2015

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December 13, 2024, Filed Under: Publications

Rapid targeted gene disruption in Bacillus anthracis.

Citation:

Saldanha RJ, Pemberton A, Shiflett P, Perutka J, Whitt JT, Ellington A, Lambowitz AM, Kramer R, Taylor D, Lamkin TJ. Rapid targeted gene disruption in Bacillus anthracis. BMC Biotechnol [Internet]. 13 :72.

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Last updated on 02/12/2015

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December 13, 2024, Filed Under: Publications

A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum.

Citation:

Mohr G, Hong W, Zhang J, Cui G-Z, Yang Y, Cui Q, Liu Y-J, Lambowitz AM. A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum. PLoS One [Internet]. 8 (7) :e69032.

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Last updated on 02/12/2015

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December 13, 2024, Filed Under: Publications

Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing.

Citation:

Mohr S, Ghanem E, Smith W, Sheeter D, Qin Y, King O, Polioudakis D, Iyer VR, Hunicke-Smith S, Swamy S, et al. Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing. RNA [Internet]. 19 (7) :958-70.

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Last updated on 02/12/2015

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December 13, 2024, Filed Under: Publications

Toward a molecular understanding of RNA remodeling by DEAD-box proteins.

Citation:

Russell R, Jarmoskaite I, Lambowitz AM. Toward a molecular understanding of RNA remodeling by DEAD-box proteins. RNA Biol [Internet]. 10 (1) :44-55.

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Last updated on 02/12/2015

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Recent Publications

Structural basis for the evolution of a domesticated group II intron–like reverse transcriptase to function in host cell DNA repair

Tagged With: 2025

Citation: Park SK, Guo M, Stamos JL, Wantae K, Lee S, Zhang YJ, Lambowitz AM. Structural basis of the evolution of a domesticated group II intron-like reverse transcriptase to function in host cell DNA repair. Biochemistry. 2025 July 29; 12231):e2504208122. Download Citation

Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers

Tagged With: 2024

Citation: Yao J, Xu H, Ferrick-Kiddie EA, Nottingham RM, Wu DC, Ares M, Lambowitz AM. Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers. PloS Genet. 2024 Sep 26; 20(9):e1011416 185 (20) :3671-3688. Download Citation

Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2

Tagged With: 2023

Citation: Cui H, Diedrich JK, Wu DC, Lim JJ, Nottingham RM, Moresco JJ, Yates JR, Blencowe BJ, Lambowitz AM, Schimmel P. Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2. Nature Cell Biology [Internet]. 25 :592–603. Download Citation cui_2023.pdf 10.53 MB Full Text News & views highlight:… read more 

Group II-like Reverse Transcriptases Function in Double Strand Break Repair

Tagged With: 2022

Citation: Park SK, Mohr G, Yao J, Russell R, Lambowitz AM. Group II-like Reverse Transcriptases Function in Double Strand Break Repair. Cell [Internet]. 185 (20) :3671-3688. Download Citation park_cell_2022.pdf 12.54 MB Full Text Cell Leading Edge Highlight: Moran, J.V. and Wilson, T.E. Reverse transcriptase meets DNA, again: Possible roles for transposable… read more 

Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling

Tagged With: 2020

Citation: Yao J, Wu DC, Nottingham RM, Lambowitz AM. Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling. eLife [Internet]. Download Citation Last updated on 09/15/2020

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