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Lambowitz Lab
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  • Research Summary
    • (A) Mobile Group II Intron, Evolutionary Ancestors of Retroviruses, Retrotransposons, and Spliceosomal Introns in Eukaryotes.
    • (B) Group II Intron Reverse Transcriptases
    • (C) Reverse Transcriptases Associated with CRISPR-Cas Systems and Other Bacterial and Organellar Reverse Transcriptases
    • (D) Thermostable Group II Intron Reverse Transcriptases (TGIRTs) and Their Applications in High Throughput RNA Sequencing (RNA-Seq).
    • (E) Analysis of Extracellular RNAs, RNA Diagnostics, and Liquid Biopsy and tRNA fragments
  • People
    • Principal Investigator
    • Assistant to Dr. Lambowitz
    • Postdoctoral Associates
    • Research Engineering/Scientist Associates
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    • Graduate Students
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Lambowitz Lab

Department of Molecular Biosciences

100 East 24th St., Stop A5000
Austin, TX 78712 (email)

Publications

December 10, 2024, Filed Under: Publications

Involvement of tyrosyl-tRNA synthetase in splicing of group I introns in Neurospora crassa mitochondria: biochemical and immunochemical analyses of splicing activity.

Citation:

Majumder AL, Akins RA, Wilkinson JG, Kelley RL, Snook AJ, Lambowitz AM. Involvement of tyrosyl-tRNA synthetase in splicing of group I introns in Neurospora crassa mitochondria: biochemical and immunochemical analyses of splicing activity. Mol Cell Biol. 9 (5) :2089-104.

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  • PubMed

December 10, 2024, Filed Under: Publications

Mutations in nuclear gene cyt-4 of Neurospora crassa result in pleiotropic defects in processing and splicing of mitochondrial RNAs.

Citation:

Dobinson KF, Henderson M, Kelley RL, Collins RA, Lambowitz AM. Mutations in nuclear gene cyt-4 of Neurospora crassa result in pleiotropic defects in processing and splicing of mitochondrial RNAs. Genetics. 123 (1) :97-108.

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  • PubMed

December 10, 2024, Filed Under: Publications

A 3′ splice site mutation in a nuclear gene encoding a mitochondrial ribosomal protein in Neurospora crassa.

Citation:

Kuiper MT, Holtrop M, Vennema H, Lambowitz AM, de Vries H. A 3′ splice site mutation in a nuclear gene encoding a mitochondrial ribosomal protein in Neurospora crassa. J Biol Chem. 263 (6) :2848-52.

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  • PubMed

December 10, 2024, Filed Under: Publications

Isolation and analysis of the Neurospora crassa Cyt-21 gene. A nuclear gene encoding a mitochondrial ribosomal protein.

Citation:

Kuiper MT, Akins RA, Holtrop M, de Vries H, Lambowitz AM. Isolation and analysis of the Neurospora crassa Cyt-21 gene. A nuclear gene encoding a mitochondrial ribosomal protein. J Biol Chem. 263 (6) :2840-7.

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  • PubMed

December 10, 2024, Filed Under: Publications

The structural gene for a phosphorus-repressible phosphate permease in Neurospora crassa can complement a mutation in positive regulatory gene nuc-1.

Citation:

Mann BJ, Akins RA, Lambowitz AM, Metzenberg RL. The structural gene for a phosphorus-repressible phosphate permease in Neurospora crassa can complement a mutation in positive regulatory gene nuc-1. Mol Cell Biol. 8 (3) :1376-9.

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  • PubMed

December 10, 2024, Filed Under: Publications

A novel reverse transcriptase activity associated with mitochondrial plasmids of Neurospora.

Citation:

Kuiper MT, Lambowitz AM. A novel reverse transcriptase activity associated with mitochondrial plasmids of Neurospora. Cell. 55 (4) :693-704.

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  • PubMed

December 4, 2024, Filed Under: Publications

Nucleotide sequence of the Varkud mitochondrial plasmid of Neurospora and synthesis of a hybrid transcript with a 5′ leader derived from mitochondrial RNA.

Citation:

Akins RA, Grant DM, Stohl LL, Bottorff DA, Nargang FE, Lambowitz AM. Nucleotide sequence of the Varkud mitochondrial plasmid of Neurospora and synthesis of a hybrid transcript with a 5′ leader derived from mitochondrial RNA. J Mol Biol. 204 (1) :1-25.

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  • PubMed

November 7, 2024, Filed Under: Publications

Molecular cloning and characterization of the cys-3 regulatory gene of Neurospora crassa.

… read more 

November 7, 2024, Filed Under: Publications

A protein required for splicing group I introns in Neurospora mitochondria is mitochondrial tyrosyl-tRNA synthetase or a derivative thereof.

… read more 

November 7, 2024, Filed Under: Publications

Mitochondrial plasmids of Neurospora and other filamentous fungi.

… read more 

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Recent Publications

Structural basis for the evolution of a domesticated group II intron–like reverse transcriptase to function in host cell DNA repair

Tagged With: 2025

Citation: Park SK, Guo M, Stamos JL, Wantae K, Lee S, Zhang YJ, Lambowitz AM. Structural basis of the evolution of a domesticated group II intron-like reverse transcriptase to function in host cell DNA repair. Biochemistry. 2025 July 29; 12231):e2504208122. Download Citation

Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers

Tagged With: 2024

Citation: Yao J, Xu H, Ferrick-Kiddie EA, Nottingham RM, Wu DC, Ares M, Lambowitz AM. Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers. PloS Genet. 2024 Sep 26; 20(9):e1011416 185 (20) :3671-3688. Download Citation

Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2

Tagged With: 2023

Citation: Cui H, Diedrich JK, Wu DC, Lim JJ, Nottingham RM, Moresco JJ, Yates JR, Blencowe BJ, Lambowitz AM, Schimmel P. Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2. Nature Cell Biology [Internet]. 25 :592–603. Download Citation cui_2023.pdf 10.53 MB Full Text News & views highlight:… read more 

Group II-like Reverse Transcriptases Function in Double Strand Break Repair

Tagged With: 2022

Citation: Park SK, Mohr G, Yao J, Russell R, Lambowitz AM. Group II-like Reverse Transcriptases Function in Double Strand Break Repair. Cell [Internet]. 185 (20) :3671-3688. Download Citation park_cell_2022.pdf 12.54 MB Full Text Cell Leading Edge Highlight: Moran, J.V. and Wilson, T.E. Reverse transcriptase meets DNA, again: Possible roles for transposable… read more 

Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling

Tagged With: 2020

Citation: Yao J, Wu DC, Nottingham RM, Lambowitz AM. Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling. eLife [Internet]. Download Citation Last updated on 09/15/2020

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