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Lambowitz Lab
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  • Research Summary
    • (A) Mobile Group II Intron, Evolutionary Ancestors of Retroviruses, Retrotransposons, and Spliceosomal Introns in Eukaryotes.
    • (B) Group II Intron Reverse Transcriptases
    • (C) Reverse Transcriptases Associated with CRISPR-Cas Systems and Other Bacterial and Organellar Reverse Transcriptases
    • (D) Thermostable Group II Intron Reverse Transcriptases (TGIRTs) and Their Applications in High Throughput RNA Sequencing (RNA-Seq).
    • (E) Analysis of Extracellular RNAs, RNA Diagnostics, and Liquid Biopsy and tRNA fragments
  • People
    • Principal Investigator
    • Assistant to Dr. Lambowitz
    • Postdoctoral Associates
    • Research Engineering/Scientist Associates
    • Research Associates
    • Graduate Students
    • Laboratory Assistants
  • Publications
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Lambowitz Lab

Department of Molecular Biosciences

100 East 24th St., Stop A5000
Austin, TX 78712 (email)

Publications

December 13, 2024, Filed Under: Publications

A Highly Proliferative Group IIC Intron from Geobacillus stearothermophilus Reveals New Features of Group II Intron Mobility and Splicing.

Citation:

Mohr G, Kang SY, Park SK, Qin Y, Grohman J, Yao J, Stamos JL, Lambowitz AM. A Highly Proliferative Group IIC Intron from Geobacillus stearothermophilus Reveals New Features of Group II Intron Mobility and Splicing. Journal of Molecular Biology [Internet]. 430 (17) :2760-2783.

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mohr_kang_2018.pdf5.16 MB

Last updated on 07/30/2018

December 13, 2024, Filed Under: Publications

Limitations of alignment-free tools in total RNA-seq quantification.

Citation:

Wu DC, Yao J, Ho KS, Lambowitz AM, Wilke CO. Limitations of alignment-free tools in total RNA-seq quantification. BMC Genomics [Internet]. 19 (1) :510.

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wu_yao_2018.pdf2.28 MB

Last updated on 07/30/2018

December 13, 2024, Filed Under: Publications

On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires.

Citation:

Silas S, Makarova KS, Shmakov S, Páez-Espino D, Mohr G, Liu Y, Davidson M, Roux S, Krishnamurthy SR, Fu B, et al. On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires. American Society for Microbiology [Internet]. 8 (4).

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December 13, 2024, Filed Under: Publications

Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching.

Citation:

Wu DC, Lambowitz AM. Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching. Scientific Reports [Internet]. 7 (8421).

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Last updated on 12/12/2017

December 13, 2024, Filed Under: Publications

Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution.

Citation:

Lambowitz AM, Belfort M. Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution. Microbiol Spectr [Internet]. 3 (1).

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Last updated on 04/28/2015

  • PubMed

December 13, 2024, Filed Under: Publications

Protein synthesis. Rqc2p and 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains.

Citation:

Shen PS, Park J, Qin Y, Li X, Parsawar K, Larson MH, Cox J, Cheng Y, Lambowitz AM, Weissman JS, et al. Protein synthesis. Rqc2p and 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains. Science [Internet]. 347 (6217) :75-8.

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Last updated on 02/12/2015

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  • DOI

December 13, 2024, Filed Under: Publications

Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis.

Citation:

Enyeart PJ, Mohr G, Ellington AD, Lambowitz AM. Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis. Mob DNA [Internet]. 5 (1) :2.

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Last updated on 02/17/2015

  • PubMed
  • DOI

December 13, 2024, Filed Under: Publications

Broad and adaptable RNA structure recognition by the human interferon-induced tetratricopeptide repeat protein IFIT5.

Citation:

Katibah GE, Qin Y, Sidote DJ, Yao J, Lambowitz AM, Collins K. Broad and adaptable RNA structure recognition by the human interferon-induced tetratricopeptide repeat protein IFIT5. Proc Natl Acad Sci U S A [Internet]. 111 (33) :12025-30.

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Last updated on 02/12/2015

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December 13, 2024, Filed Under: Publications

The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons.

Citation:

Hong W, Zhang J, Feng Y, Mohr G, Lambowitz AM, Cui G-Z, Liu Y-J, Cui Q. The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons. Biotechnol Biofuels [Internet]. 7 :80.

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Last updated on 02/12/2015

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December 13, 2024, Filed Under: Publications

Evolution of RNA-protein interactions: non-specific binding led to RNA splicing activity of fungal mitochondrial tyrosyl-tRNA synthetases.

Citation:

Lamech LT, Mallam AL, Lambowitz AM. Evolution of RNA-protein interactions: non-specific binding led to RNA splicing activity of fungal mitochondrial tyrosyl-tRNA synthetases. PLoS Biol [Internet]. 12 (12) :e1002028.

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Last updated on 02/12/2015

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Recent Publications

Structural basis for the evolution of a domesticated group II intron–like reverse transcriptase to function in host cell DNA repair

Tagged With: 2025

Citation: Park SK, Guo M, Stamos JL, Wantae K, Lee S, Zhang YJ, Lambowitz AM. Structural basis of the evolution of a domesticated group II intron-like reverse transcriptase to function in host cell DNA repair. Biochemistry. 2025 July 29; 12231):e2504208122. Download Citation

Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers

Tagged With: 2024

Citation: Yao J, Xu H, Ferrick-Kiddie EA, Nottingham RM, Wu DC, Ares M, Lambowitz AM. Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers. PloS Genet. 2024 Sep 26; 20(9):e1011416 185 (20) :3671-3688. Download Citation

Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2

Tagged With: 2023

Citation: Cui H, Diedrich JK, Wu DC, Lim JJ, Nottingham RM, Moresco JJ, Yates JR, Blencowe BJ, Lambowitz AM, Schimmel P. Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2. Nature Cell Biology [Internet]. 25 :592–603. Download Citation cui_2023.pdf 10.53 MB Full Text News & views highlight:… read more 

Group II-like Reverse Transcriptases Function in Double Strand Break Repair

Tagged With: 2022

Citation: Park SK, Mohr G, Yao J, Russell R, Lambowitz AM. Group II-like Reverse Transcriptases Function in Double Strand Break Repair. Cell [Internet]. 185 (20) :3671-3688. Download Citation park_cell_2022.pdf 12.54 MB Full Text Cell Leading Edge Highlight: Moran, J.V. and Wilson, T.E. Reverse transcriptase meets DNA, again: Possible roles for transposable… read more 

Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling

Tagged With: 2020

Citation: Yao J, Wu DC, Nottingham RM, Lambowitz AM. Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling. eLife [Internet]. Download Citation Last updated on 09/15/2020

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