General Themes and Goals of our Research Group:
The general theme of our lab is discovery and characterization of RNA regulators for uncovering complex cellular phenotypes. A major application of our work is the manipulation of RNA regulatory systems to engineer more robust cellular phenotypes that can better withstand natural environmental or chemical stresses (like those imposed by the incorporation of heterologous metabolic pathways). Some of the critical tools used in our lab include biochemical approaches, systems biology approaches, genetic studies, large-scale “omics” data, molecular engineering, synthetic biology and bioinformatics. We also collaborate with a number of top scientists to incorporate structural biology, molecular dynamics modeling, kinetic simulations and other types of approaches into our work.
Areas of Research:
1. Characterization of non-coding RNA regulatory networks.
It is becoming clear that RNAs function in the context of highly intricate and interrelated networks. In our lab, we have developed a number of tools to characterize the molecular details and diversity of these interactions as well as their ultimate effects in regulating the versatility of RNA functioning.
2. RNA conformational changes in vivo and the relevance of these structural features for gene targeting approaches.
RNAs highly depend on their conformational flexibility for stability and function. One of our interests is to understand specific mechanisms by which RNAs are stabilized in vivo by other biological molecules and to apply this knowledge to the engineering of a wide range of RNAs with robust and controllable properties.
3. Engineering synthetic RNA elements.
4. Characterizing RNA-mediated regulation pathways that are affected by various sources of oxidative stresses to build early disease diagnostics.
*Research overview and portions of figures created using BioRender.com