Requirement for the sample FAQs:

Sample preparation form MicroED:  Kindly fill out this form either by editing it directly in Adobe Acrobat or by printing it and completing it manually

Sample Type: Small-molecule or protein microcrystals (<1 µm)

Purity: >95% recommended, pure NMR, HR-MS or PXRD required.

Quantity: Provide a few µL of slurry or several mg of crystals in a clean vial, (preferably HPLC vial).

Crystal Size: 200–500 nm ideal

Crystal Shape: Well-formed, minimal aggregation

Solvent: Volatile preferred (ethanol, acetone), avoid viscous/non-volatile solvents

Storage: 4°C for proteins, avoid repeated freeze-thaw

Contamination: Keep it free from: dust, grease, debris, plastic, cotton, filter papers, hair etc. Do not use Eppendorf containers, falcon tubes etc, plastic screw caps.

Grid Type: Quantifoil, lacey carbon, holey carbon

Application: 1–3 µL on grid, blot excess carefully

Vitrification: Plunge-freeze in liquid ethane (proteins), air-dry if compatible (small molecules)

Labeling: Sample ID, date, optional crystal sketch/photo

Shipping: Dry ice for frozen proteins, room temperature for small molecules, leak-proof and cushioned.

Samples will be measured at the CBRL facility on Thermo Fisher Glacios.