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Liu Lab

The University of Texas at Austin College of Pharmacy

2006-2004

  1. Hong, L.; Zhao, Z.; Liu, H.-w. “Characterization SpnQ from the Spinosyn Biosynthetic Pathway ofSaccharopolyspora spinosa: Mechanistic and Evolutionary Implications for C-3 Deoxygenation in Deoxysugar Biosynthesis.” J. Am. Chem. Soc. 2006, 128, 14262-14263.
  2. Melancon, C. E.; Thibodeaux, C.; Liu, H.-w. “Glyco-stripping and Glyco-swapping.” JAC Chem. Biol. 2006,1, 499-504.
  3. Yan, F.; Munos, J. W.; Liu, H.-w. “Biosynthesis of Fosfomycin: Re-examination and Re-confirmation of A Unique Fe(II) and NAD(P)H-dependent Epoxidation Reaction.” Biochemistry 2006, 45, 11473-11481.
  4. Zhang, H.; Zhou, Y.; Bao, H.; Liu, H.-w. “Vi Antigen Biosynthesis in Salmonella typhi: Characterization of UDP-N-acetylglucosamine C-6 Dehydrogenase (TviB), and UDP-N-acetylglucosaminuronic Acid C-4 Epimerase (TviC).” Biochemistry 2006, 45, 8163-8173.
  5. Kao, C.-L.; Borisova, S. A.; Kim, H. J.; Liu, H.-w. “Linear Aglycones are the Substrates for Glycosyltransferase DesVII in Methymycin Biosynthesis: Analysis and Implications.” J. Am. Chem. Soc..2006, 128, 5606-5607.
  6. Smith, P.; Lin, A.; Szu, P.-H.; Liu, H.-w.; Tsai, S.-C. “Biosynthesis of 3,6-Dideoxy-hexoses: Crystallization and X-ray Crystallography of CDP-6-deoxy-L-threo-D-glycero-4-hexulose 3-Dehydrase (E1) for Ascarylose Biosynthesis.” Acta Cryst. 2006, F62, 231-234.
  7. Takahashi, H,; Liu, Y.-n.; Liu, H.-w. “A Two-Stage One-Pot Enzymatic Synthesis of TDP-L-mycarose from Thymidine and Glucose-1-phosphate.” J. Am. Chem. Soc. 2006, 128, 1432-1433.
  8. Borisova, S. A.; Zhang, C.; Zhang, H.; Takahashi, H.; Wong, A.; Thorson, J. S.; Liu, H.-w. “Substrate Specificity of the Macrolide Glycosylating Enzyme Pair DesVII/DesVIII: Opportunities, Limitations, and Mechanistic Hypotheses.” Angew. Chem. Int. Ed. 2006, 45, 2748-2753.
  9. Kao, C.-l.; Kittleman, W.; Zhang, H.; Seto, H.; Liu, H.-w. “Stereochemical Analysis of Isopentenyl Diphosphate Isomerase Type II from Staphylococcus aureus Using Chemically Synthesized (S)- and (R)-[2-2H]Isopentenyl Diphosphates.” Org. Lett., 2005, 7, 5677-5680.
  10. Melancon C. E., III; Yu, W.-l. A.; Liu, H.-w. “TDP-Mycaminose Biosynthetic Pathway Revised and Conversion of Desosamine Pathway to Mycaminose Pathway with One Gene.” J. Am. Chem. Soc. 2005, 127, 12240-12241.
  11. Yan, F.; Li, T.; Lipscomb, J. D.; Liu, A.; Liu, H.-w. “Site-directed Mutagenesis and Spectroscopic Studies on the Active Site of (S)-2-Hydroxypropylphosphonic Acid Epoxidase: An Unusual 2-His-1-Carboxylate Iron-Binding Triad.” Arch. Biochem. Biopys. 2005, 442, 82-91.
  12. Szu, P.-h.; He, X.; Zhao, L.; Liu, H.-w. “Biosynthesis of TDP-D-Desosamine: Elucidation of a New Strategy for C-4 Deoxygenation.” Angew. Chem. Int. Ed. 2005, 44, 6742-6746.
  13. Takahashi, H.; Liu, Y.-n.; Chen, H.; Liu, H.-w. “Biosynthesis of TDP-Mycarose: The Specificity of a Single Enzyme Governs the Outcome of the Pathway.” J. Am. Chem. Soc. 2005, 127, 9340-9341.
  14. Higgins, L.; Yan, F.; Liu, P.; Liu, H.-w.; Drennan, C. L. “Structural Insight into the Biosynthesis of the Antibiotics Fosfomycin by a Mononuclear Iron Enzyme.” Nature, 2005, 437, 838-844..
  15. Zhao, Z.; Hong, L.; Liu, H.-w. “Characterization of Protein Encoded by spnR from Spinosyn Gene Cluster ofSaccharopolyspora spinosa: Mechanistic Implications of Forosamine Biosynthesis.” J. Am. Chem. Soc. 2005,127, 7692-7693.
  16. Alam, J.; Beyer, N.; Liu, H.-w. “Biosynthesis of Colitose: Expression, Purification and Mechanistic Characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose Synthase (ColC).” Biochemistry 2004, 43, 16450-16460.
  17. Melancon, C. E. III.; Takahashi, H.; Liu, H.-w. “Characterization of tylM2/tylM3 and mycB/mydC Pairs Required for Efficient Glycosyltransfer in Macrolide Antibiotic Biosynthesis.” J. Am. Chem. Soc. 2004, 126, 16726-16727.
  18. Wong, A.; Munos, J. W.; Devasthali, V.; Johnson, K. A.; Liu, H.-w. “Studies of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase: Synthesis and Evaluation of Fluorinated Substrate Analogues.” Org. Lett. 2004,6, 3625-3628.
  19. Retey, J.; Liu, H.-w. “Mechanisms: The Elucidation of Fasinating New Enzyme-catalyzed Reactions Continues.” Cur. Opin. Chem. Biol. 2004, 8, 451-453.
  20. Pongdee, R.; Liu, H.-w. “Elucidation of Enzyme Mechanisms Using Fluorinated Substrate Analogues.”Bioorg. Chem. 2004, 32, 393-437.
  21. Agnihotri, G.; Liu, Y.-n.; Paschal, B.; Liu, H.-w. “Identification of An Unusual [2Fe-2S]-Binding Motif in the Dehydrase Responsible for C-3 Deoxygenation in the Biosynthesis of 3,6-Dideoxyhexoses.” Biochemistry2004, 43, 14265-14274.
  22. Karthikeyan, S.; Zhou, Q.; Zhao, Z.; Kao, C.-L.; Tao, Z.; Robinson, H.; Liu, H.-w.; Zhang, H. “Structural Analysis of Pseudomonas 1-Aminocyclopropane-1-carboxylate Deaminase Complexes: Insight Into the Mechanism of a Unique Pyridoxal-5′-Phosphate-Dependent Cyclopropane Ring-Opening Reaction.”Biochemistry 2004, 43, 13328-13339.
  23. Liu, P.; Mehn, M. P.; Yan, F.; Zhao, Z.; Que, L.; Liu, H.-w. “Oxygenase Activity in the Self-Hydroxylation of (S)-2-Hydroxypropylphosphonic Acid Epoxidase Involved in Fosfomycin Biosynthesis.” J. Am. Chem. Soc.2004, 126, 10306-10312.
  24. Borisova, S.; Zhao, L.; Melancon, C.; Kao, C.-L.; Liu, H.-w. “Characterization of the Glycosyltransferase Activity of DesVII: Analysis of and Implication for the Biosynthesis of Macrolide Antibiotics.” J. Am. Chem. Soc. 2004, 126, 6534-6535.
  25. Karthikeyan, S.; Zhao, Z.; Kao, C.-l.; Zhou, Q.; Tao, Z.; Zhang, H.; Liu, H.-w. “Structural Analysis of 1-Aminocyclopropane-1-carboxylate Deaminase: Observation of an Aminyl Intermediate and Indentification of Tyr294 as an Active-site Nucleophile.” Angew. Chem. Int. Ed. Engl. 2004, 43, 3425-3429 (Angew. Chem.2004, 116, 3507-3511).
  26. Vogan, E. M.; Bellamacina, C.; He, X.; Liu, H.-w.; Ringe, D.; Petsko, G. A. “Crystal Structure at 1.8 Angstrom Resolution of CDP-D-glucose 4,6-Dehydratase from Yersinia pseudotuberculosis.” Biochemistry2004, 43, 3057-3067.
  27. Rocklin, A. M.; Kato, K.; Liu, H.-w.; Que, L., Jr.; Lipscomb, J. D. “Mechanistic Studies of 1-Aminocyclopropane-1-carboxylic Acid Oxidase: Single Turnover Reaction.” J. Bioinorg. Chem. 2004, 9, 171-182.