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2021-2019

  1. Joen, B.-s.; Huang, T.-Y.; Ruszczycky, M. W.; Choi, S.-h.; Kim, N.; Franklin, J. L.; Hung, S.-C.; Liu, H.-w. “Byproduct Formation During the Biosynthesis of Spinosyn A and Evidence for an Enzymatic Interplay to Prevent its Formation.” Tetrahedron 2021, in press. [NIHMSID1759956]
  2. Choi, S.-h.; Jeon, B.; Kim, N.; Wu, H.-H.; Ko, T.-P.; Ruszczycky, M. W.; Isiorho, E. A.; Liu, Y.-n.; Keatinge-Clay, A. T.; Tsai, M.-D.; Liu, H.-w. “Evidence for an Enzyme Catalyzed Rauhut-Currier Reaction during the Biosynthesis of Spinosyn A.” J. Am. Chem. Soc. 2021, 143, 20291-20295. [PMC8665079]
  3. Besandre, R.; Chen, Z.; David, I.; Zhang, J.; Ruszczycky, M. W.; Liu, A.; Liu, H.-w. “HygY is a Twitch Radical SAM Epimerase with Latent Dehydrogenase Activity Revealed upon Mutation of a Single Cysteine Residue.” J. Am. Chem. Soc. 2021, 143, 15152-15158. [PMC8611816]
  4. Ruszczycky, M. W.; Liu, H.-w. “Distinguishing Concerted versus Stepwise Mechanisms Using Isotope Effects on Isotope Effects.” Biochemistry 2021, 60, 3416-3418. [PMC8612942]
  5. Ren, D.; Kim, M.; Wang, S.-A.; Liu, H.-w. “Identification of a Pyrrole Intermediate Which Undergoes C-Glycosidation and Autoxidation to Yield the Final Product in Showdomycin Biosynthesis.” Angew. Chem. Int. Ed. 2021, 60, 17148-17154. [PMC8292215]
  6. Yeh, Y.-C.; Kim, H. J.; Liu, H.-w. “Mechanistic Investigation of 1,2-Diol Dehydration of Paromamine Catalyzed by the Radical S-Adenosyl-L-methionine Enzyme AprD4.” J. Am. Chem. Soc. 2021, 143, 5038-5043. [PMC8045149]
  7. Zhong, A.; Lee, Y.-H.; Liu, Y.-n.; Liu, H.-w. “Biosynthesis of Oxetanocin A Includes a B12-dependent Radical SAM Enzyme Able to Catalyze both Oxidative Ring Contraction and the Demethylation of SAM.” Biochemistry 2021, 60, 537-546. [PMC7904626]
  8. Wang, S.-A.; Lin, C.-I.; Zhang, J.; Ushimaru, R.; Sasaki, E.; Liu, H.-w. “Studies of Lincosamide Formation Complete the Biosynthetic Pathway for Lincomycin A.” Proc. Natl. Acad. Sci. USA 2020, 117, 24794-24801.
  9. Zhang, J.; Fan, P.-H.; Lin, G.-M.; Chang, W.-C.; Liu, H.-w. “Recent Progress in the Biosynthesis of Carbohydrate-Containing Natural Products.” In Comprehensive Chemistry of Natural Product Chemistry, Liu, H-w.; Begley, T., Eds.; Elsvier: New York;. 2020, 336-392.
  10. Ren, D.; Ruszczycky, M. W.; Ko, Y.; Wang, S.-A.; Ogasawara, Y.; Kim, M.; Liu, H.-w. “Characterization of the Coformycin Biosynthetic Gene Cluster in Streptomyces kaniharaensis.” Proc. Natl. Aca. Sci. USA. 2020, 117, 10265-10270. [PMC7229688]
  11. Ushimaru, R.; Chen, Z.; Zhao, H.; Fan, P.-h.; Liu, H.-w. “Identification of the Enzymes Mediating the Maturation of the Sery-tRNA Synthetase Inhibitor SB-217452 during the Biosynthesis of Albomycins.” Angew. Chem. Int. Ed. 2019, 59, 3558-3562. [PMC7138408]
  12. Ruszczycky, M. W.; Liu, H.-w. “Measurement of Net Rate Constants from Enzyme Progress Curves without Numerical Curve Fitting.” Biochemistry. 2019, 58, 4950-4956. [PMC6910778].
  13. Ren, D.; Wang, S.-A.; Ko, Y.; Geng, Y.; Ogasawara, Y.; Liu, H.-w. “Identification of the C-Glycoside Synthases during Biosynthesis of the Pyrazole-C-Nucleosides Formycin and Pyrazofurin.” Angew. Chem. Int. Ed. 2019, 58, 16512-16516. [PMC6911263].
  14. Romo, A.; Ikeuchi, H.; Shiraishi, T.; Lin, G.-M.; Geng, Y.; Lee, Y.-H.; Liem, P. H.; Ma, T.; Ogasawara, Y.; Shin-Ya, K.; Nishiyama, M.; Kuzuyama, T.; Liu, H.-w. “Miharamycins and Amipurimycin Biosynthetic Gene Clusters: Unravelling the Origins of 2-Aminopurinyl Peptidyl Nucleoside Antibiotics.” J. Am. Chem. Soc. 2019, 141, 14152-14159. [PMC6774755].
  15. Wang, S.-A.; Ko, Y.; Zheng, J.; Geng, Y.; Ren, D.; Ogasawara, Y.; Irani, S.; Zhang, Y.; Liu, H.-w. “Identification of the Formycin A Biosynthetic Gene Cluster from Streptomyces kaniharaensis Illustrates the Interplay Between Biological Pyrazolopyrimidine Formation and de novo Purine Biosynthesis.” J. Am. Chem. Soc. 2019, 141, 6127-6131. [NIHMSID 1021733]
  16. Pan, H.-X.; Chen, Z.; Zeng, T.; Jin, W.-B.; Geng, Y.; Lin, G.-M.; Zhao, J.; Li, W.-T.; Xiong, Z.; Huang, S.-X.; Zhai, X.; Liu, H.-w.; Tang, G.-L. “Elucidation of the Herbicidin Tailoring Pathway Offers New Insights into Its Structural Diversity.” Org. Lett. 2019, 21, 1374-1378. [NIHMSID1020766]
  17. Ushimaru, R.; Liu, H.-w. “Biosynthetic Origin of the Atypical Stereochemistry in the Thioheptose Core of Albomycin Nucleoside Antibiotics.” J. Am. Chem. Soc. 2019, 141, 2211-2214. [PMC6446896]
  18. Ushimaru, R.; Ruszczycky, M. W.; Liu, H.-w. “Changes in Regiospecificity of H-atom Abstraction During the Hydroxylation and Cyclization Reactions Catalyzed by Hyoscyamine 6β-Hydroxylase.” J. Am. Chem. Soc. 2019, 141, 1062-1066. [NIHMSID 1020764]
  19. Thibodeaux, C. J.; Chang, W.-C.; Liu, H.-w. “Unraveling Flavoenzyme Reaction Mechanisms Using Flavin Analogues and Linear Free Energy Relationships.” Meth. Enzymol. 2019, submitted
  20. Ban, Y. H.; Song, M. C.; Hwang, J.-y.; Shin, H.-l.; Kim, H. J.; Hong, S. K.; Lee, N. J.; Park, J. W.; Cha, S.-S.; Liu, H.-w.; Yoon, Y. J. “Complete Reconstitution of the Diverse Pathways of Gentamicin B Biosynthesis.” Nature Chem. Biol. 2019, 15, 295-303. [NIHMSID 1512665]